Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda)
M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV–visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N′-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7270/ http://psasir.upm.edu.my/id/eprint/7270/1/Factors%20affecting%20the%20nucleolytic%20cleavage%20of%20DNA%20by.pdf |
| _version_ | 1848840548862918656 |
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| author | Ng, Chew Hee Ong, Alan Han Kiat Kong, Chiak Wu Tan, Kong Wai Raja Abdul Rahman, Raja Noor Zaliha Mohd Yamin, Bohari Ng, Seik Weng |
| author_facet | Ng, Chew Hee Ong, Alan Han Kiat Kong, Chiak Wu Tan, Kong Wai Raja Abdul Rahman, Raja Noor Zaliha Mohd Yamin, Bohari Ng, Seik Weng |
| author_sort | Ng, Chew Hee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV–visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N′-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na2H2EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change. |
| first_indexed | 2025-11-15T07:29:06Z |
| format | Article |
| id | upm-7270 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:29:06Z |
| publishDate | 2006 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-72702016-04-26T08:48:35Z http://psasir.upm.edu.my/id/eprint/7270/ Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) Ng, Chew Hee Ong, Alan Han Kiat Kong, Chiak Wu Tan, Kong Wai Raja Abdul Rahman, Raja Noor Zaliha Mohd Yamin, Bohari Ng, Seik Weng M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV–visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N′-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na2H2EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change. Elsevier 2006-11-13 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7270/1/Factors%20affecting%20the%20nucleolytic%20cleavage%20of%20DNA%20by.pdf Ng, Chew Hee and Ong, Alan Han Kiat and Kong, Chiak Wu and Tan, Kong Wai and Raja Abdul Rahman, Raja Noor Zaliha and Mohd Yamin, Bohari and Ng, Seik Weng (2006) Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda). Polyhedron, 25 (16). pp. 3118-3126. ISSN 0277-5387; ESSN: 1873-3719 http://www.sciencedirect.com/science/article/pii/S0277538706002567 10.1016/j.poly.2006.05.020 |
| spellingShingle | Ng, Chew Hee Ong, Alan Han Kiat Kong, Chiak Wu Tan, Kong Wai Raja Abdul Rahman, Raja Noor Zaliha Mohd Yamin, Bohari Ng, Seik Weng Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) |
| title | Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) |
| title_full | Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) |
| title_fullStr | Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) |
| title_full_unstemmed | Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) |
| title_short | Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda) |
| title_sort | factors affecting the nucleolytic cleavage of dna by (n,n′-ethylenendiaminediacetato)metal(ii) complexes, m(edda). crystal structure of co(edda) |
| url | http://psasir.upm.edu.my/id/eprint/7270/ http://psasir.upm.edu.my/id/eprint/7270/ http://psasir.upm.edu.my/id/eprint/7270/ http://psasir.upm.edu.my/id/eprint/7270/1/Factors%20affecting%20the%20nucleolytic%20cleavage%20of%20DNA%20by.pdf |