Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration
Large amount of sodium hydroxide (NaOH) is consumed to remove the protein content in chitin biomass during deproteinization. However, excessive NaOH concentration used might lead to the reduction of cost effectiveness during chitin extraction. Hence, the present study aimed to extract and evaluate t...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72691/ http://psasir.upm.edu.my/id/eprint/72691/1/Extraction%20and%20physicochemical%20characterization%20of%20chitin%20and%20chitosan%20from%20Zophobas%20morio%20larvae%20in%20varying%20sodium%20hydroxide%20concentration.pdf |
| _version_ | 1848857179948318720 |
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| author | Soon, Chu Yong Tee, Yee Bond Tan, Choon Hui Abdul Talib, Rosnita Abdan, Khalina |
| author_facet | Soon, Chu Yong Tee, Yee Bond Tan, Choon Hui Abdul Talib, Rosnita Abdan, Khalina |
| author_sort | Soon, Chu Yong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Large amount of sodium hydroxide (NaOH) is consumed to remove the protein content in chitin biomass during deproteinization. However, excessive NaOH concentration used might lead to the reduction of cost effectiveness during chitin extraction. Hence, the present study aimed to extract and evaluate the physicochemical properties of chitin and chitosan isolated from superworm (Zophobas morio) larvae using 0.5 M–2.0 M of NaOH. The extracted chitin and chitosan were subjected to Fourier Transform Infrared Spectroscopy (FT-IR), elemental analysis, Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC) and X-ray Diffraction (XRD). The 0.5 M NaOH treatment resulted in the highest yield of chitin (5.43%), but produced the lowest yield (65.84%) of chitosan. The extracted chitin samples had relatively high degree of acetylation (DA) (82.39%-101.39%). Both chitin and chitosan showed smooth surface with tiny pores. The extracted chitin samples were confirmed as α-chitin based on the FT-IR and TGA. The chitin samples were amorphous with low degree of crystallinity. From TGA, the Chitosan 3 extracted was partially deacetylated. Both DPPH radical scavenging and ferric-chelating assay showed positive correlation with DD of chitosan isolates. However, the chitosan isolates were not fully dissolved, resulting in lower radical scavenging and ferric-chelating ability compared to commercial chitosan. |
| first_indexed | 2025-11-15T11:53:27Z |
| format | Article |
| id | upm-72691 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:53:27Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-726912021-01-05T05:26:07Z http://psasir.upm.edu.my/id/eprint/72691/ Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration Soon, Chu Yong Tee, Yee Bond Tan, Choon Hui Abdul Talib, Rosnita Abdan, Khalina Large amount of sodium hydroxide (NaOH) is consumed to remove the protein content in chitin biomass during deproteinization. However, excessive NaOH concentration used might lead to the reduction of cost effectiveness during chitin extraction. Hence, the present study aimed to extract and evaluate the physicochemical properties of chitin and chitosan isolated from superworm (Zophobas morio) larvae using 0.5 M–2.0 M of NaOH. The extracted chitin and chitosan were subjected to Fourier Transform Infrared Spectroscopy (FT-IR), elemental analysis, Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC) and X-ray Diffraction (XRD). The 0.5 M NaOH treatment resulted in the highest yield of chitin (5.43%), but produced the lowest yield (65.84%) of chitosan. The extracted chitin samples had relatively high degree of acetylation (DA) (82.39%-101.39%). Both chitin and chitosan showed smooth surface with tiny pores. The extracted chitin samples were confirmed as α-chitin based on the FT-IR and TGA. The chitin samples were amorphous with low degree of crystallinity. From TGA, the Chitosan 3 extracted was partially deacetylated. Both DPPH radical scavenging and ferric-chelating assay showed positive correlation with DD of chitosan isolates. However, the chitosan isolates were not fully dissolved, resulting in lower radical scavenging and ferric-chelating ability compared to commercial chitosan. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72691/1/Extraction%20and%20physicochemical%20characterization%20of%20chitin%20and%20chitosan%20from%20Zophobas%20morio%20larvae%20in%20varying%20sodium%20hydroxide%20concentration.pdf Soon, Chu Yong and Tee, Yee Bond and Tan, Choon Hui and Abdul Talib, Rosnita and Abdan, Khalina (2018) Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration. International Journal of Biological Macromolecules, 108. 135 - 142. ISSN 0141-8130 https://www.sciencedirect.com/science/article/abs/pii/S0141813017327630 10.1016/j.ijbiomac.2017.11.138 |
| spellingShingle | Soon, Chu Yong Tee, Yee Bond Tan, Choon Hui Abdul Talib, Rosnita Abdan, Khalina Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration |
| title | Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration |
| title_full | Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration |
| title_fullStr | Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration |
| title_full_unstemmed | Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration |
| title_short | Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration |
| title_sort | extraction and physicochemical characterization of chitin and chitosan from zophobas morio larvae in varying sodium hydroxide concentration |
| url | http://psasir.upm.edu.my/id/eprint/72691/ http://psasir.upm.edu.my/id/eprint/72691/ http://psasir.upm.edu.my/id/eprint/72691/ http://psasir.upm.edu.my/id/eprint/72691/1/Extraction%20and%20physicochemical%20characterization%20of%20chitin%20and%20chitosan%20from%20Zophobas%20morio%20larvae%20in%20varying%20sodium%20hydroxide%20concentration.pdf |