Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate
Conversion of tilapia by-products through enzymatic hydrolysis is a promising alternative to produce a fish protein hydrolysate (FPH). Tilapia by-product (TB) protein hydrolysate consists of peptide mixtures in various sizes. Recovery of small-sized peptides is increasingly becoming a priority due t...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72648/ http://psasir.upm.edu.my/id/eprint/72648/1/Evaluation%20on%20performance%20of.pdf |
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| author | Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah |
| author_facet | Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah |
| author_sort | Roslan, Jumardi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Conversion of tilapia by-products through enzymatic hydrolysis is a promising alternative to produce a fish protein hydrolysate (FPH). Tilapia by-product (TB) protein hydrolysate consists of peptide mixtures in various sizes. Recovery of small-sized peptides is increasingly becoming a priority due to their special characteristics, which can provide an excellent physiological functions. Ultrafiltration (UF) membrane is an effective and suitable tool for fractionating small size peptides in a large scale. The objective of this study was to evaluate the membrane performance based on the permeate flux and peptide transmission. The fractionation of TB protein hydrolysate was performed through a dead-end UF membrane (10 and 5 kDa) with the effect of stirring speed (0–600 rpm), pH (3, 5, 7, 8 and 9) and salt concentration (NaCl; 0 M, 0.2 M, 0.4 M, and 0.6 M) at varies pressure (1.0, 1.5, 2.0, 2.5 and 3.0 bar). It was found that the transmembrane pressure of 2.5 bar, stirring speed of 600 rpm, pH 8 and without addition of NaCl turned out to be the best parameters which gave permeate flux of 53 L/m2h (10 kDa) and 27 L/m2h (5 kDa), while for the peptide transmission, the value of 87.33% (10 kDa) and 36.11% (5kDa) was obtained. This study indicates that by adding salt, it has lowered the permeate flux and peptide transmission. Finally, through a well-controlled of operating and physicochemical parameters, it was possible to produce peptides with sizes lower than 1000 Da. |
| first_indexed | 2025-11-15T11:53:17Z |
| format | Article |
| id | upm-72648 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:53:17Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-726482020-11-20T15:09:32Z http://psasir.upm.edu.my/id/eprint/72648/ Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah Conversion of tilapia by-products through enzymatic hydrolysis is a promising alternative to produce a fish protein hydrolysate (FPH). Tilapia by-product (TB) protein hydrolysate consists of peptide mixtures in various sizes. Recovery of small-sized peptides is increasingly becoming a priority due to their special characteristics, which can provide an excellent physiological functions. Ultrafiltration (UF) membrane is an effective and suitable tool for fractionating small size peptides in a large scale. The objective of this study was to evaluate the membrane performance based on the permeate flux and peptide transmission. The fractionation of TB protein hydrolysate was performed through a dead-end UF membrane (10 and 5 kDa) with the effect of stirring speed (0–600 rpm), pH (3, 5, 7, 8 and 9) and salt concentration (NaCl; 0 M, 0.2 M, 0.4 M, and 0.6 M) at varies pressure (1.0, 1.5, 2.0, 2.5 and 3.0 bar). It was found that the transmembrane pressure of 2.5 bar, stirring speed of 600 rpm, pH 8 and without addition of NaCl turned out to be the best parameters which gave permeate flux of 53 L/m2h (10 kDa) and 27 L/m2h (5 kDa), while for the peptide transmission, the value of 87.33% (10 kDa) and 36.11% (5kDa) was obtained. This study indicates that by adding salt, it has lowered the permeate flux and peptide transmission. Finally, through a well-controlled of operating and physicochemical parameters, it was possible to produce peptides with sizes lower than 1000 Da. Elsevier 2018-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72648/1/Evaluation%20on%20performance%20of.pdf Roslan, Jumardi and Mustapa Kamal, Siti Mazlina and Md. Yunos, Khairul Faezah and Abdullah, Norhafizah (2018) Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate. Separation and Purification Technology, 195. 21 - 29. ISSN 1383-5866 https://www.sciencedirect.com/science/article/pii/S1383586617319949 10.1016/j.seppur.2017.11.020 |
| spellingShingle | Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| title | Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| title_full | Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| title_fullStr | Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| title_full_unstemmed | Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| title_short | Evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| title_sort | evaluation on performance of dead-end ultrafiltration membrane in fractionating tilapia by-product protein hydrolysate |
| url | http://psasir.upm.edu.my/id/eprint/72648/ http://psasir.upm.edu.my/id/eprint/72648/ http://psasir.upm.edu.my/id/eprint/72648/ http://psasir.upm.edu.my/id/eprint/72648/1/Evaluation%20on%20performance%20of.pdf |