A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene
An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205...
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| Format: | Article |
| Language: | English |
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Elsevier
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7246/ http://psasir.upm.edu.my/id/eprint/7246/1/A%20novel%20organic%20solvent%20tolerant%20lipase%20from%20Bacillus%20sphaericus%20205y%20extracellular%20expression%20of%20a%20novel%20OST-lipase%20gene.pdf |
| _version_ | 1848840542252695552 |
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| author | Sulong, Moohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Sulong, Moohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Sulong, Moohamad Ropaning |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205y lipase revealed homogeneity on denaturing gel electrophoresis and the molecular mass was at approximately 30 kDa. The optimum pH for the purified 205y lipase was 7.0–8.0 and its stability showed a broad range of pH value between pH 5.0 to 13.0 at 37 °C. The purified 205y lipase exhibited an optimum temperature of 55 °C. The activity of the purified lipase was stimulated in the presence of Ca2+ and Mg2+. Ethylenediaminetetraacetic acid (EDTA) has no effect on its activity; however inhibition was observed with phenylmethane sulfonoyl fluoride (PMSF) a serine hydrolase inhibitor. Organic solvents such as dimethylsulfoxide (DMSO), methanol, p-xylene and n-decane enhanced the activity. Studies on the effect of oil showed that the lipase was most active in the presence of tricaprin (C10). The lipase exhibited 1,3 positional specificity. |
| first_indexed | 2025-11-15T07:29:00Z |
| format | Article |
| id | upm-7246 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:29:00Z |
| publishDate | 2006 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-72462016-09-26T07:58:55Z http://psasir.upm.edu.my/id/eprint/7246/ A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene Sulong, Moohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205y lipase revealed homogeneity on denaturing gel electrophoresis and the molecular mass was at approximately 30 kDa. The optimum pH for the purified 205y lipase was 7.0–8.0 and its stability showed a broad range of pH value between pH 5.0 to 13.0 at 37 °C. The purified 205y lipase exhibited an optimum temperature of 55 °C. The activity of the purified lipase was stimulated in the presence of Ca2+ and Mg2+. Ethylenediaminetetraacetic acid (EDTA) has no effect on its activity; however inhibition was observed with phenylmethane sulfonoyl fluoride (PMSF) a serine hydrolase inhibitor. Organic solvents such as dimethylsulfoxide (DMSO), methanol, p-xylene and n-decane enhanced the activity. Studies on the effect of oil showed that the lipase was most active in the presence of tricaprin (C10). The lipase exhibited 1,3 positional specificity. Elsevier 2006-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7246/1/A%20novel%20organic%20solvent%20tolerant%20lipase%20from%20Bacillus%20sphaericus%20205y%20extracellular%20expression%20of%20a%20novel%20OST-lipase%20gene.pdf Sulong, Moohamad Ropaning and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2006) A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene. Protein Expression and Purification, 49 (2). pp. 190-195. ISSN 1046-5928; ESSN: 1096-0279 10.1016/j.pep.2006.04.015 |
| spellingShingle | Sulong, Moohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene |
| title | A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene |
| title_full | A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene |
| title_fullStr | A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene |
| title_full_unstemmed | A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene |
| title_short | A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene |
| title_sort | novel organic solvent tolerant lipase from bacillus sphaericus 205y: extracellular expression of a novel ost-lipase gene |
| url | http://psasir.upm.edu.my/id/eprint/7246/ http://psasir.upm.edu.my/id/eprint/7246/ http://psasir.upm.edu.my/id/eprint/7246/1/A%20novel%20organic%20solvent%20tolerant%20lipase%20from%20Bacillus%20sphaericus%20205y%20extracellular%20expression%20of%20a%20novel%20OST-lipase%20gene.pdf |