An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization
The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K pr...
| Main Authors: | Raja Abdul Rahman, Raja Noor Zaliha, Lee, Poh Geok, Basri, Mahiran, Salleh, Abu Bakar |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2006
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/7245/ http://psasir.upm.edu.my/id/eprint/7245/1/1An%20organic%20solvent.pdf |
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