Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila
Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African ca...
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| Format: | Article |
| Language: | English |
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Universiti Teknologi Malaysia Press
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72443/ http://psasir.upm.edu.my/id/eprint/72443/1/Effect%20of%20putative%20probiont%20Enterococcus%20hirae%20on%20the%20hematological%20parameters%20.pdf |
| _version_ | 1848857120204652544 |
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| author | Hamid, Nur Hidayahanum Mohd Daud, Hassan Srisapoome, Prapansak Abu Hassim, Hasliza Mohd Yusoff, Md Sabri Kayansamruaj, Pattanapon Hamdan, Ruhil Hayati Abdul Sani, Siti Fairus Abu Bakar, Siti Nadia |
| author_facet | Hamid, Nur Hidayahanum Mohd Daud, Hassan Srisapoome, Prapansak Abu Hassim, Hasliza Mohd Yusoff, Md Sabri Kayansamruaj, Pattanapon Hamdan, Ruhil Hayati Abdul Sani, Siti Fairus Abu Bakar, Siti Nadia |
| author_sort | Hamid, Nur Hidayahanum |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African catfish, Clarias gariepinus during pre- and post-challenge with aquatic pathogen, Aeromonas hydrophila. The probiotics were previously isolated from vegetable wastes (mung bean sprouts, Vigna radiate and cucumber, Cucumis sativus) which have been fermented for 7 days. The experimental fish (270 tails) with an average weight of 5.13 ± 1.03 g were distributed and divided randomly into i) control (30 tails), fed with commercial diet ii) E1 (30 tails), fed with diets supplemented with 108 CFU/ml of E. faecium isolated from fermented cucumber, iii) E2 (30 tails), fed with 108 CFU/ml of E. faecium isolated from fermented mung bean sprouts. The feeding trial was conducted for 50 days. All experimental groups were then challenged with A. hydrophila (1.5 × 106 CFU/mL) via intraperitoneal injection on day 51. Prior to challenge, blood samples were collected from five fish randomly selected from each group on the day 51 (pre-challenge). After 72 hours of post-challenge, blood samples were again collected from five fish from each groups. The hematological parameters such as total erythrocyte count (RBC), total leucocyte count (WBC), packed cell volume (PCV), hemoglobin (Hb), the derived blood indices of mean corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) were examined. Hematological profiles of pre- and post-challenge infected juvenile catfish were compared with the control groups. The RBC, Hb, WBC, PCV, MCV and MCHC of fish fed with probiotics showed higher significant difference (P<0.05) as compared to control groups during pre- and post-challenge of pathogen. The high level of RBC and WBC during pre- and post-challenge showed the capability of the probiotics to improve the immune response of juvenile African catfish and thus increased the fish disease resistance against A. hydrophila infection. The result suggested that E. faecium could be used effectively as a probiotic in aquaculture. |
| first_indexed | 2025-11-15T11:52:30Z |
| format | Article |
| id | upm-72443 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:52:30Z |
| publishDate | 2018 |
| publisher | Universiti Teknologi Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-724432020-06-11T02:03:59Z http://psasir.upm.edu.my/id/eprint/72443/ Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila Hamid, Nur Hidayahanum Mohd Daud, Hassan Srisapoome, Prapansak Abu Hassim, Hasliza Mohd Yusoff, Md Sabri Kayansamruaj, Pattanapon Hamdan, Ruhil Hayati Abdul Sani, Siti Fairus Abu Bakar, Siti Nadia Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African catfish, Clarias gariepinus during pre- and post-challenge with aquatic pathogen, Aeromonas hydrophila. The probiotics were previously isolated from vegetable wastes (mung bean sprouts, Vigna radiate and cucumber, Cucumis sativus) which have been fermented for 7 days. The experimental fish (270 tails) with an average weight of 5.13 ± 1.03 g were distributed and divided randomly into i) control (30 tails), fed with commercial diet ii) E1 (30 tails), fed with diets supplemented with 108 CFU/ml of E. faecium isolated from fermented cucumber, iii) E2 (30 tails), fed with 108 CFU/ml of E. faecium isolated from fermented mung bean sprouts. The feeding trial was conducted for 50 days. All experimental groups were then challenged with A. hydrophila (1.5 × 106 CFU/mL) via intraperitoneal injection on day 51. Prior to challenge, blood samples were collected from five fish randomly selected from each group on the day 51 (pre-challenge). After 72 hours of post-challenge, blood samples were again collected from five fish from each groups. The hematological parameters such as total erythrocyte count (RBC), total leucocyte count (WBC), packed cell volume (PCV), hemoglobin (Hb), the derived blood indices of mean corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) were examined. Hematological profiles of pre- and post-challenge infected juvenile catfish were compared with the control groups. The RBC, Hb, WBC, PCV, MCV and MCHC of fish fed with probiotics showed higher significant difference (P<0.05) as compared to control groups during pre- and post-challenge of pathogen. The high level of RBC and WBC during pre- and post-challenge showed the capability of the probiotics to improve the immune response of juvenile African catfish and thus increased the fish disease resistance against A. hydrophila infection. The result suggested that E. faecium could be used effectively as a probiotic in aquaculture. Universiti Teknologi Malaysia Press 2018 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/72443/1/Effect%20of%20putative%20probiont%20Enterococcus%20hirae%20on%20the%20hematological%20parameters%20.pdf Hamid, Nur Hidayahanum and Mohd Daud, Hassan and Srisapoome, Prapansak and Abu Hassim, Hasliza and Mohd Yusoff, Md Sabri and Kayansamruaj, Pattanapon and Hamdan, Ruhil Hayati and Abdul Sani, Siti Fairus and Abu Bakar, Siti Nadia (2018) Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila. Malaysian Journal of Fundamental and Applied Sciences, 14 (3). 423 - 428. ISSN 2289-5981; ESSN: 2289-599X https://mjfas.utm.my/index.php/mjfas/article/view/1097 |
| spellingShingle | Hamid, Nur Hidayahanum Mohd Daud, Hassan Srisapoome, Prapansak Abu Hassim, Hasliza Mohd Yusoff, Md Sabri Kayansamruaj, Pattanapon Hamdan, Ruhil Hayati Abdul Sani, Siti Fairus Abu Bakar, Siti Nadia Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila |
| title | Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila |
| title_full | Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila |
| title_fullStr | Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila |
| title_full_unstemmed | Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila |
| title_short | Effect of putative probiont Enterococcus hirae on the haematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila |
| title_sort | effect of putative probiont enterococcus hirae on the haematological parameters of juvenile african catfish, clarias gariepinus (burchell, 1822) during pre- and post-challenge against aeromonas hydrophila |
| url | http://psasir.upm.edu.my/id/eprint/72443/ http://psasir.upm.edu.my/id/eprint/72443/ http://psasir.upm.edu.my/id/eprint/72443/1/Effect%20of%20putative%20probiont%20Enterococcus%20hirae%20on%20the%20hematological%20parameters%20.pdf |