A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
The surface antigen (HBsAg) of hepatitis B virus (HBV) is conformational and generally evokes protective humoral immune response in human. A disulfide constrained, andom heptapeptide library displayed on the coat protein III of filamentous bacteriophage M13 was employed to select specific ligands th...
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| Format: | Article |
| Language: | English English |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/724/ http://psasir.upm.edu.my/id/eprint/724/1/A%20Phage%20Displayed%20Cyclic%20Peptide%20That%20Interacts%20Tightly%20With%20the%20Immunodominant%20Region%20of%20Hepatitis%20B%20Surface%20Antigen..pdf |
| _version_ | 1848838847863980032 |
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| author | Wen, Siang Tan Geok, Hun Tan Yusoff, Khatijah Heng, Fong Seow |
| author_facet | Wen, Siang Tan Geok, Hun Tan Yusoff, Khatijah Heng, Fong Seow |
| author_sort | Wen, Siang Tan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The surface antigen (HBsAg) of hepatitis B virus (HBV) is conformational and generally evokes protective humoral immune response in human. A disulfide constrained, andom heptapeptide library displayed on the coat protein III of filamentous bacteriophage M13 was employed to select specific ligands that interact with HBsAg subtype ad. Fusion phages carrying the amino acid sequence ETGAKPH and other related sequences were isolated. The binding site of peptide ETGAKPH was located on the immunodominant region of HBsAg. An equilibrium binding assays in solution showed that the phage binds tightly to HBsAg with a relative dissociation constant(krel D) of 2.9 ± 0.9 nM. The phage bearing this peptide has the potential to be used as a diagnostic reagent and two assays for detecting HBsAg in blood samples are described. |
| first_indexed | 2025-11-15T07:02:04Z |
| format | Article |
| id | upm-724 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:02:04Z |
| publishDate | 2005 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-7242015-10-22T02:43:05Z http://psasir.upm.edu.my/id/eprint/724/ A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. Wen, Siang Tan Geok, Hun Tan Yusoff, Khatijah Heng, Fong Seow The surface antigen (HBsAg) of hepatitis B virus (HBV) is conformational and generally evokes protective humoral immune response in human. A disulfide constrained, andom heptapeptide library displayed on the coat protein III of filamentous bacteriophage M13 was employed to select specific ligands that interact with HBsAg subtype ad. Fusion phages carrying the amino acid sequence ETGAKPH and other related sequences were isolated. The binding site of peptide ETGAKPH was located on the immunodominant region of HBsAg. An equilibrium binding assays in solution showed that the phage binds tightly to HBsAg with a relative dissociation constant(krel D) of 2.9 ± 0.9 nM. The phage bearing this peptide has the potential to be used as a diagnostic reagent and two assays for detecting HBsAg in blood samples are described. Elsevier 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/724/1/A%20Phage%20Displayed%20Cyclic%20Peptide%20That%20Interacts%20Tightly%20With%20the%20Immunodominant%20Region%20of%20Hepatitis%20B%20Surface%20Antigen..pdf Wen, Siang Tan and Geok, Hun Tan and Yusoff, Khatijah and Heng, Fong Seow (2005) A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. Journal of Clinical Virology, 34 (1). pp. 35-41. ISSN 1386-6532 http://dx.doi.org/10.1016/j.jcv.2005.01.007 English |
| spellingShingle | Wen, Siang Tan Geok, Hun Tan Yusoff, Khatijah Heng, Fong Seow A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. |
| title | A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. |
| title_full | A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. |
| title_fullStr | A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. |
| title_full_unstemmed | A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. |
| title_short | A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. |
| title_sort | phage – displayed cyclic peptide that interacts tightly with the immunodominant region of hepatitis b surface antigen. |
| url | http://psasir.upm.edu.my/id/eprint/724/ http://psasir.upm.edu.my/id/eprint/724/ http://psasir.upm.edu.my/id/eprint/724/1/A%20Phage%20Displayed%20Cyclic%20Peptide%20That%20Interacts%20Tightly%20With%20the%20Immunodominant%20Region%20of%20Hepatitis%20B%20Surface%20Antigen..pdf |