Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
Aim: This study aimed to evaluate the effect of edible bird’s nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF...
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| Format: | Article |
| Language: | English |
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Veterinary World
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72369/ http://psasir.upm.edu.my/id/eprint/72369/1/Edible%20bird%E2%80%99s%20nest%20impact%20on%20rats%E2%80%99%20uterine%20histomorphology.pdf |
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| author | Albishtue, Abdulla A. Degu, Nurhusien Yimer Abu Bakar @ Zakaria, Md Zuki Haron, Abd Wahid Yusoff, Rosnina Assi, Mohammed A. Almhanawi, Bahaa H. |
| author_facet | Albishtue, Abdulla A. Degu, Nurhusien Yimer Abu Bakar @ Zakaria, Md Zuki Haron, Abd Wahid Yusoff, Rosnina Assi, Mohammed A. Almhanawi, Bahaa H. |
| author_sort | Albishtue, Abdulla A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aim: This study aimed to evaluate the effect of edible bird’s nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and steroid receptors. Materials and Methods: Twenty-four Sprague Dawley rats were equally distributed into the following four groups: G1 (control), G2, G3, and G4 represented the groups treated with EBN at graded concentrations of 0, 30, 60, and 120 mg/kg body weight (BW) per day for 8 weeks, respectively. During the experimental period, the BW of each rat was recorded
weekly. At the proestrus stage of estrous cycle, blood samples were collected from the hearts of anesthetized rats that were later sacrificed. The uteri were removed for histological and immunohistochemical analyses. Results: The EBN-treated groups showed an increase in the weights and lengths of uteri as compared to the control. Results showed that relative to G1 and G2, G3 and G4 exhibited proliferation in their uterine luminal and glandular epithelia
and uterine glands, and up-regulated expressions of EGF, REGF, VEGF, PCNA, and progesterone receptor, and estrogen
receptor in their uteri. The EBN increased the antioxidant (AO) and total AO capacities and reduced the oxidative stress
(OS) levels in non-pregnant rats. Conclusion: Findings of this study revealed that EBN promotes proliferation of the uterine structures as evidenced by the upregulation of the expressions of steroid receptors,EGF, REGF, VEGF, and PCNA in the uterus and increased in the plasma concentrations of AO and reduced levels of OS. |
| first_indexed | 2025-11-15T11:52:12Z |
| format | Article |
| id | upm-72369 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:52:12Z |
| publishDate | 2018 |
| publisher | Veterinary World |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-723692020-05-27T03:35:07Z http://psasir.upm.edu.my/id/eprint/72369/ Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level Albishtue, Abdulla A. Degu, Nurhusien Yimer Abu Bakar @ Zakaria, Md Zuki Haron, Abd Wahid Yusoff, Rosnina Assi, Mohammed A. Almhanawi, Bahaa H. Aim: This study aimed to evaluate the effect of edible bird’s nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and steroid receptors. Materials and Methods: Twenty-four Sprague Dawley rats were equally distributed into the following four groups: G1 (control), G2, G3, and G4 represented the groups treated with EBN at graded concentrations of 0, 30, 60, and 120 mg/kg body weight (BW) per day for 8 weeks, respectively. During the experimental period, the BW of each rat was recorded weekly. At the proestrus stage of estrous cycle, blood samples were collected from the hearts of anesthetized rats that were later sacrificed. The uteri were removed for histological and immunohistochemical analyses. Results: The EBN-treated groups showed an increase in the weights and lengths of uteri as compared to the control. Results showed that relative to G1 and G2, G3 and G4 exhibited proliferation in their uterine luminal and glandular epithelia and uterine glands, and up-regulated expressions of EGF, REGF, VEGF, PCNA, and progesterone receptor, and estrogen receptor in their uteri. The EBN increased the antioxidant (AO) and total AO capacities and reduced the oxidative stress (OS) levels in non-pregnant rats. Conclusion: Findings of this study revealed that EBN promotes proliferation of the uterine structures as evidenced by the upregulation of the expressions of steroid receptors,EGF, REGF, VEGF, and PCNA in the uterus and increased in the plasma concentrations of AO and reduced levels of OS. Veterinary World 2018-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72369/1/Edible%20bird%E2%80%99s%20nest%20impact%20on%20rats%E2%80%99%20uterine%20histomorphology.pdf Albishtue, Abdulla A. and Degu, Nurhusien Yimer and Abu Bakar @ Zakaria, Md Zuki and Haron, Abd Wahid and Yusoff, Rosnina and Assi, Mohammed A. and Almhanawi, Bahaa H. (2018) Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level. Veterinary World, 11 (1). 71 - 79. ISSN 0972-8988; ESSN: 2231-0916 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813515/ 10.14202/vetworld.2018.71-79 |
| spellingShingle | Albishtue, Abdulla A. Degu, Nurhusien Yimer Abu Bakar @ Zakaria, Md Zuki Haron, Abd Wahid Yusoff, Rosnina Assi, Mohammed A. Almhanawi, Bahaa H. Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| title | Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| title_full | Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| title_fullStr | Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| title_full_unstemmed | Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| title_short | Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| title_sort | edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level |
| url | http://psasir.upm.edu.my/id/eprint/72369/ http://psasir.upm.edu.my/id/eprint/72369/ http://psasir.upm.edu.my/id/eprint/72369/ http://psasir.upm.edu.my/id/eprint/72369/1/Edible%20bird%E2%80%99s%20nest%20impact%20on%20rats%E2%80%99%20uterine%20histomorphology.pdf |