Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male croco...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72303/ http://psasir.upm.edu.my/id/eprint/72303/1/Digital%20massage%20for%20semen%20collection.pdf |
| _version_ | 1848857086250713088 |
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| author | Wan Jaafar, Wan Nor Fitri Haron, Abd Wahid Tengku Azizan, Tengku Rinalfi Putra Yusoff, Rosnina Raj, Dana Yawah, Donny Ab. Latip, Mohd Qayyum Ab Aziz, Abdul Malek |
| author_facet | Wan Jaafar, Wan Nor Fitri Haron, Abd Wahid Tengku Azizan, Tengku Rinalfi Putra Yusoff, Rosnina Raj, Dana Yawah, Donny Ab. Latip, Mohd Qayyum Ab Aziz, Abdul Malek |
| author_sort | Wan Jaafar, Wan Nor Fitri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male crocodiles kept in the same enclosure were physically restrained for semen collection. The collection was done in January 2015 during crocodiles mating season. Mean (± SEM) seminal volume, mass movement, pH, sperm concentration, general motility, forward movement, rotating and vibrating movements measured were 0.55 ± 0.12 ml, 2.75 ± 0.48, 7.13 ± 0.24, 396.88 ± 43.41 × 106 sperm/ml, 45 ± 17.56%, 37.13 ± 5.12%, 9.56 ± 5.38% and 53.13 ± 1.92% respectively. The most important sperm abnormalities that requires further investigation is the cytoplasmic droplets which was 28.25 ± 2.29%. Sperm motility trend was best preserved in extender made up of 0.01 M PBS with 5% egg yolk. Sperm stored 4 °C in this extender had survived longer than the other two extender which was 11 days. There is a unique trend in the spike of spermatozoa motility post 48 h in chilled condition however the cause of such pattern is still unknown. Semen collection by digital manipulation of the penis was adequate in obtaining semen and the semen characteristics of the estuarine crocodile are described. |
| first_indexed | 2025-11-15T11:51:57Z |
| format | Article |
| id | upm-72303 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:51:57Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-723032020-05-03T07:11:21Z http://psasir.upm.edu.my/id/eprint/72303/ Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) Wan Jaafar, Wan Nor Fitri Haron, Abd Wahid Tengku Azizan, Tengku Rinalfi Putra Yusoff, Rosnina Raj, Dana Yawah, Donny Ab. Latip, Mohd Qayyum Ab Aziz, Abdul Malek Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male crocodiles kept in the same enclosure were physically restrained for semen collection. The collection was done in January 2015 during crocodiles mating season. Mean (± SEM) seminal volume, mass movement, pH, sperm concentration, general motility, forward movement, rotating and vibrating movements measured were 0.55 ± 0.12 ml, 2.75 ± 0.48, 7.13 ± 0.24, 396.88 ± 43.41 × 106 sperm/ml, 45 ± 17.56%, 37.13 ± 5.12%, 9.56 ± 5.38% and 53.13 ± 1.92% respectively. The most important sperm abnormalities that requires further investigation is the cytoplasmic droplets which was 28.25 ± 2.29%. Sperm motility trend was best preserved in extender made up of 0.01 M PBS with 5% egg yolk. Sperm stored 4 °C in this extender had survived longer than the other two extender which was 11 days. There is a unique trend in the spike of spermatozoa motility post 48 h in chilled condition however the cause of such pattern is still unknown. Semen collection by digital manipulation of the penis was adequate in obtaining semen and the semen characteristics of the estuarine crocodile are described. Elsevier 2018-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72303/1/Digital%20massage%20for%20semen%20collection.pdf Wan Jaafar, Wan Nor Fitri and Haron, Abd Wahid and Tengku Azizan, Tengku Rinalfi Putra and Yusoff, Rosnina and Raj, Dana and Yawah, Donny and Ab. Latip, Mohd Qayyum and Ab Aziz, Abdul Malek (2018) Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus). Aquaculture, 483. 169 - 172. ISSN 0044-8486 https://www.sciencedirect.com/science/article/abs/pii/S0044848617315600 10.1016/j.aquaculture.2017.10.026 |
| spellingShingle | Wan Jaafar, Wan Nor Fitri Haron, Abd Wahid Tengku Azizan, Tengku Rinalfi Putra Yusoff, Rosnina Raj, Dana Yawah, Donny Ab. Latip, Mohd Qayyum Ab Aziz, Abdul Malek Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) |
| title | Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) |
| title_full | Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) |
| title_fullStr | Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) |
| title_full_unstemmed | Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) |
| title_short | Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) |
| title_sort | digital massage for semen collection, evaluation and extension in malaysian estuarine crocodile (crocodylus porosus) |
| url | http://psasir.upm.edu.my/id/eprint/72303/ http://psasir.upm.edu.my/id/eprint/72303/ http://psasir.upm.edu.my/id/eprint/72303/ http://psasir.upm.edu.my/id/eprint/72303/1/Digital%20massage%20for%20semen%20collection.pdf |