Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
Context: Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. Objective: To investigate the anti-proliferative potential of D. linear...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72284/ http://psasir.upm.edu.my/id/eprint/72284/1/Dicranopteris%20linearis%20extract%20inhibits%20the%20proliferation%20of%20human%20breast%20cancer%20cell%20line.pdf |
| _version_ | 1848857081914851328 |
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| author | Baharuddin, Aifaa Akmal Roosli, Rushduddin Al Jufri Zakaria, Zainul Amiruddin Md Tohid, Siti Farah |
| author_facet | Baharuddin, Aifaa Akmal Roosli, Rushduddin Al Jufri Zakaria, Zainul Amiruddin Md Tohid, Siti Farah |
| author_sort | Baharuddin, Aifaa Akmal |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Context: Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. Objective: To investigate the anti-proliferative potential of D. linearis leaves and determine possible mechanistic pathways. Materials and methods: MTT assay was used to determine the cytotoxic effects of D. linearis methanol (MEDL) and petroleum ether (PEEDL) extracts at concentrations of 100, 50, 25, 12.5, 6.25 and 3.125 µg/mL against a panel of cancer cell lines (breast [MCF-7 and MDA-MB-231], cervical [HeLa], colon [HT-29], hepatocellular [HepG2] and lung [A549]), as compared to negative (untreated) and positive [5-fluorouracil (5-FU)-treated] control groups. Mouse fibroblast cells (3T3) were used as normal cells. The mode of cell death was examined using morphological analysis via acridine orange (AO) and propidium iodide (PI) double staining. Cell cycle arrest was determined using flow cytometer, followed by annexin V-PI apoptosis detection kit. Results: MEDL demonstrated the most significant growth inhibition against MDA-MB-231 cells (IC50 22.4 µg/mL). PEEDL showed no cytotoxic effect. Induction of apoptosis by MEDL was evidenced via morphological analysis and acridine orange propidium iodide staining. MEDL could induce S phase cell cycle arrest after 72 h of incubation. Early apoptosis induction in MDA-MB-231 cells was confirmed by annexin V-FITC and PI staining. Significant increase in apoptotic cells were detected after 24 h of treatment with 15.07% cells underwent apoptosis, and the amount escalated to 18.24% with prolonged 48 h incubation.
Conclusions: MEDL has potential as a potent cytotoxic agent against MDA-MB-231 adenocarcinoma. |
| first_indexed | 2025-11-15T11:51:53Z |
| format | Article |
| id | upm-72284 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:51:53Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-722842020-04-30T08:18:35Z http://psasir.upm.edu.my/id/eprint/72284/ Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis Baharuddin, Aifaa Akmal Roosli, Rushduddin Al Jufri Zakaria, Zainul Amiruddin Md Tohid, Siti Farah Context: Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. Objective: To investigate the anti-proliferative potential of D. linearis leaves and determine possible mechanistic pathways. Materials and methods: MTT assay was used to determine the cytotoxic effects of D. linearis methanol (MEDL) and petroleum ether (PEEDL) extracts at concentrations of 100, 50, 25, 12.5, 6.25 and 3.125 µg/mL against a panel of cancer cell lines (breast [MCF-7 and MDA-MB-231], cervical [HeLa], colon [HT-29], hepatocellular [HepG2] and lung [A549]), as compared to negative (untreated) and positive [5-fluorouracil (5-FU)-treated] control groups. Mouse fibroblast cells (3T3) were used as normal cells. The mode of cell death was examined using morphological analysis via acridine orange (AO) and propidium iodide (PI) double staining. Cell cycle arrest was determined using flow cytometer, followed by annexin V-PI apoptosis detection kit. Results: MEDL demonstrated the most significant growth inhibition against MDA-MB-231 cells (IC50 22.4 µg/mL). PEEDL showed no cytotoxic effect. Induction of apoptosis by MEDL was evidenced via morphological analysis and acridine orange propidium iodide staining. MEDL could induce S phase cell cycle arrest after 72 h of incubation. Early apoptosis induction in MDA-MB-231 cells was confirmed by annexin V-FITC and PI staining. Significant increase in apoptotic cells were detected after 24 h of treatment with 15.07% cells underwent apoptosis, and the amount escalated to 18.24% with prolonged 48 h incubation. Conclusions: MEDL has potential as a potent cytotoxic agent against MDA-MB-231 adenocarcinoma. Taylor & Francis 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72284/1/Dicranopteris%20linearis%20extract%20inhibits%20the%20proliferation%20of%20human%20breast%20cancer%20cell%20line.pdf Baharuddin, Aifaa Akmal and Roosli, Rushduddin Al Jufri and Zakaria, Zainul Amiruddin and Md Tohid, Siti Farah (2018) Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis. Pharmaceutical Biology, 56 (1). 422 - 432. ISSN 1388-0209; ESSN: 1744-5116 https://www.tandfonline.com/doi/abs/10.1080/13880209.2018.1495748 10.1080/13880209.2018.1495748 |
| spellingShingle | Baharuddin, Aifaa Akmal Roosli, Rushduddin Al Jufri Zakaria, Zainul Amiruddin Md Tohid, Siti Farah Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis |
| title | Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis |
| title_full | Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis |
| title_fullStr | Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis |
| title_full_unstemmed | Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis |
| title_short | Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis |
| title_sort | dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (mda-mb-231) via induction of s-phase arrest and apoptosis |
| url | http://psasir.upm.edu.my/id/eprint/72284/ http://psasir.upm.edu.my/id/eprint/72284/ http://psasir.upm.edu.my/id/eprint/72284/ http://psasir.upm.edu.my/id/eprint/72284/1/Dicranopteris%20linearis%20extract%20inhibits%20the%20proliferation%20of%20human%20breast%20cancer%20cell%20line.pdf |