D-galactose and aluminium chloride induced rat model with cognitive impairments
Cognitive impairments and cholinergic dysfunctions have been well reported in old age disorders including Alzheimer’s disease (AD). d-galactose (D-gal) has been reported as a senescence agent while aluminium act as a neurotoxic metal, but little is known about their combined effects at different dos...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72276/ http://psasir.upm.edu.my/id/eprint/72276/1/D-galactose%20and%20aluminium%20chloride%20induced%20rat%20model%20with%20cognitive%20impairments.pdf |
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| author | Musa Chiroma, Samaila Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Baharuldin, Mohamad Taufik Hidayat Amom, Zulkhairi |
| author_facet | Musa Chiroma, Samaila Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Baharuldin, Mohamad Taufik Hidayat Amom, Zulkhairi |
| author_sort | Musa Chiroma, Samaila |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cognitive impairments and cholinergic dysfunctions have been well reported in old age disorders including Alzheimer’s disease (AD). d-galactose (D-gal) has been reported as a senescence agent while aluminium act as a neurotoxic metal, but little is known about their combined effects at different doses. The aim of this study was to establish an animal model with cognitive impairments by comparing the effects of different doses of co-administrated D-gal and aluminium chloride (AlCl3). In this study male albino wistar rats were administered with D-gal 60 mg/kg.bwt intra peritoneally (I.P) injected and AlCl3 (100, 200, or 300 mg/kg.bwt.) was orally administered once daily for 10 consecutive weeks. Performance of the rats were evaluated through behavioural assessments; Morris water maze (MWM) and open field tests (OFT); histopathological examination was performed on the hippocampus; moreover biochemical measurements of acetylcholinesterase (AChE) and hyperphosphorylated tau protein (p-tau) were examined. The results of this experiment on rats treated with D-gal 60 + AlCl3 200 mg/kg.bwt showed near ideal cognitive impairments. The rats exhibited an obvious memory and learning deficits, marked neuronal loss in hippocampus, showed increase in AChE activities and high expression of p-tau within the tissues of the brain. This study concludes that D-gal 60 + AlCl3 200 mg/kg.bwt as the ideal dose for mimicking AD like cognitive impairments in albino wistar rats. It is also crucial to understand the pathogenesis of this neurodegenerative disease and for drug discovery. |
| first_indexed | 2025-11-15T11:51:51Z |
| format | Article |
| id | upm-72276 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:51:51Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-722762020-04-28T07:00:26Z http://psasir.upm.edu.my/id/eprint/72276/ D-galactose and aluminium chloride induced rat model with cognitive impairments Musa Chiroma, Samaila Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Baharuldin, Mohamad Taufik Hidayat Amom, Zulkhairi Cognitive impairments and cholinergic dysfunctions have been well reported in old age disorders including Alzheimer’s disease (AD). d-galactose (D-gal) has been reported as a senescence agent while aluminium act as a neurotoxic metal, but little is known about their combined effects at different doses. The aim of this study was to establish an animal model with cognitive impairments by comparing the effects of different doses of co-administrated D-gal and aluminium chloride (AlCl3). In this study male albino wistar rats were administered with D-gal 60 mg/kg.bwt intra peritoneally (I.P) injected and AlCl3 (100, 200, or 300 mg/kg.bwt.) was orally administered once daily for 10 consecutive weeks. Performance of the rats were evaluated through behavioural assessments; Morris water maze (MWM) and open field tests (OFT); histopathological examination was performed on the hippocampus; moreover biochemical measurements of acetylcholinesterase (AChE) and hyperphosphorylated tau protein (p-tau) were examined. The results of this experiment on rats treated with D-gal 60 + AlCl3 200 mg/kg.bwt showed near ideal cognitive impairments. The rats exhibited an obvious memory and learning deficits, marked neuronal loss in hippocampus, showed increase in AChE activities and high expression of p-tau within the tissues of the brain. This study concludes that D-gal 60 + AlCl3 200 mg/kg.bwt as the ideal dose for mimicking AD like cognitive impairments in albino wistar rats. It is also crucial to understand the pathogenesis of this neurodegenerative disease and for drug discovery. Elsevier 2018-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72276/1/D-galactose%20and%20aluminium%20chloride%20induced%20rat%20model%20with%20cognitive%20impairments.pdf Musa Chiroma, Samaila and Mohd Moklas, Mohamad Aris and Mat Taib, Che Norma and Baharuldin, Mohamad Taufik Hidayat and Amom, Zulkhairi (2018) D-galactose and aluminium chloride induced rat model with cognitive impairments. Biomedicine and Pharmacotherapy, 103. 1602 - 1608. ISSN 0753-3322; ESSN: 1950-6007 https://www.sciencedirect.com/science/article/abs/pii/S075333221736674X 10.1016/j.biopha.2018.04.152 |
| spellingShingle | Musa Chiroma, Samaila Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Baharuldin, Mohamad Taufik Hidayat Amom, Zulkhairi D-galactose and aluminium chloride induced rat model with cognitive impairments |
| title | D-galactose and aluminium chloride induced rat model with cognitive impairments |
| title_full | D-galactose and aluminium chloride induced rat model with cognitive impairments |
| title_fullStr | D-galactose and aluminium chloride induced rat model with cognitive impairments |
| title_full_unstemmed | D-galactose and aluminium chloride induced rat model with cognitive impairments |
| title_short | D-galactose and aluminium chloride induced rat model with cognitive impairments |
| title_sort | d-galactose and aluminium chloride induced rat model with cognitive impairments |
| url | http://psasir.upm.edu.my/id/eprint/72276/ http://psasir.upm.edu.my/id/eprint/72276/ http://psasir.upm.edu.my/id/eprint/72276/ http://psasir.upm.edu.my/id/eprint/72276/1/D-galactose%20and%20aluminium%20chloride%20induced%20rat%20model%20with%20cognitive%20impairments.pdf |