Development of polyclonal antibody against clenbuterol for immunoassay application
Development of an immunoassay for clenbuterol (CLB) detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity...
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| Format: | Article |
| Language: | English |
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MDPI
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72268/ http://psasir.upm.edu.my/id/eprint/72268/1/Development%20of%20polyclonal%20antibody.pdf |
| _version_ | 1848857077685944320 |
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| author | A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran |
| author_facet | A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran |
| author_sort | A. Talib, Nurul Ain |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Development of an immunoassay for clenbuterol (CLB) detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity column and the antibody purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The activities of purified antibody were evaluated based on high antibody titer determined from enzyme-linked immunosorbent assay (ELISA). The sensitivity and selectivity of this antibody was evaluated and exhibits negligible cross-reactivity to antibiotics other than β-agonist families. Evaluation of the antibody as bioreceptor in immunoassay was performed using direct competitive ELISA and exhibited linear calibration plot (R2 = 0.9484). The antibody was used to detect the content of CLB in spiked milk samples and the recovery of more than 92% indicating significant performance as bioreceptor for the development of a rapid and simple immunoassay. |
| first_indexed | 2025-11-15T11:51:49Z |
| format | Article |
| id | upm-72268 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:51:49Z |
| publishDate | 2018 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-722682020-04-27T06:55:34Z http://psasir.upm.edu.my/id/eprint/72268/ Development of polyclonal antibody against clenbuterol for immunoassay application A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran Development of an immunoassay for clenbuterol (CLB) detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity column and the antibody purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The activities of purified antibody were evaluated based on high antibody titer determined from enzyme-linked immunosorbent assay (ELISA). The sensitivity and selectivity of this antibody was evaluated and exhibits negligible cross-reactivity to antibiotics other than β-agonist families. Evaluation of the antibody as bioreceptor in immunoassay was performed using direct competitive ELISA and exhibited linear calibration plot (R2 = 0.9484). The antibody was used to detect the content of CLB in spiked milk samples and the recovery of more than 92% indicating significant performance as bioreceptor for the development of a rapid and simple immunoassay. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72268/1/Development%20of%20polyclonal%20antibody.pdf A. Talib, Nurul Ain and Salam, Faridah and Sulaiman, Yusran (2018) Development of polyclonal antibody against clenbuterol for immunoassay application. Molecules, 23 (4). pp. 1-14. ISSN 1420-3049; ESSN: 1420-3049 https://www.mdpi.com/1420-3049/23/4/789 10.3390/molecules23040789 |
| spellingShingle | A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran Development of polyclonal antibody against clenbuterol for immunoassay application |
| title | Development of polyclonal antibody against clenbuterol for immunoassay application |
| title_full | Development of polyclonal antibody against clenbuterol for immunoassay application |
| title_fullStr | Development of polyclonal antibody against clenbuterol for immunoassay application |
| title_full_unstemmed | Development of polyclonal antibody against clenbuterol for immunoassay application |
| title_short | Development of polyclonal antibody against clenbuterol for immunoassay application |
| title_sort | development of polyclonal antibody against clenbuterol for immunoassay application |
| url | http://psasir.upm.edu.my/id/eprint/72268/ http://psasir.upm.edu.my/id/eprint/72268/ http://psasir.upm.edu.my/id/eprint/72268/ http://psasir.upm.edu.my/id/eprint/72268/1/Development%20of%20polyclonal%20antibody.pdf |