Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of South-East Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome count...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72220/ http://psasir.upm.edu.my/id/eprint/72220/1/Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20.pdf |
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| author | Midin, Mohd Razik Nordin, Mohd Shukor Madon, Maria Salleh @ Japri, Mohd Nazre Goh, Hoe Han Mohd Noor, Normah |
| author_facet | Midin, Mohd Razik Nordin, Mohd Shukor Madon, Maria Salleh @ Japri, Mohd Nazre Goh, Hoe Han Mohd Noor, Normah |
| author_sort | Midin, Mohd Razik |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of South-East Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74 to 110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C = 2.5 pg) used as standard, G. mangostana genome size was found to be 2C = 6.00 ± 0.17 pg. Meanwhile, a genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing; k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA = 0.9780 × 109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana is between 6.00 and 6.05 pg. |
| first_indexed | 2025-11-15T11:51:39Z |
| format | Article |
| id | upm-72220 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:51:39Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-722202020-04-14T14:50:02Z http://psasir.upm.edu.my/id/eprint/72220/ Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses Midin, Mohd Razik Nordin, Mohd Shukor Madon, Maria Salleh @ Japri, Mohd Nazre Goh, Hoe Han Mohd Noor, Normah Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of South-East Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74 to 110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C = 2.5 pg) used as standard, G. mangostana genome size was found to be 2C = 6.00 ± 0.17 pg. Meanwhile, a genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing; k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA = 0.9780 × 109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana is between 6.00 and 6.05 pg. Taylor & Francis 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72220/1/Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20.pdf Midin, Mohd Razik and Nordin, Mohd Shukor and Madon, Maria and Salleh @ Japri, Mohd Nazre and Goh, Hoe Han and Mohd Noor, Normah (2018) Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses. Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics, 71 (1). 35 - 44. ISSN 0008-7114; ESSN: 2165-5391 https://www.tandfonline.com/doi/abs/10.1080/00087114.2017.1403762 10.1080/00087114.2017.1403762 |
| spellingShingle | Midin, Mohd Razik Nordin, Mohd Shukor Madon, Maria Salleh @ Japri, Mohd Nazre Goh, Hoe Han Mohd Noor, Normah Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title | Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_full | Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_fullStr | Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_full_unstemmed | Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_short | Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_sort | determination of the chromosome number and genome size of garcinia mangostana l. via cytogenetics, flow cytometry and k-mer analyses |
| url | http://psasir.upm.edu.my/id/eprint/72220/ http://psasir.upm.edu.my/id/eprint/72220/ http://psasir.upm.edu.my/id/eprint/72220/ http://psasir.upm.edu.my/id/eprint/72220/1/Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20.pdf |