CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separa...
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| Format: | Article |
| Language: | English |
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Biotechnical Faculty, University of Ljubljana
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72153/ http://psasir.upm.edu.my/id/eprint/72153/1/CUPRAC%20assay-guided%20profiling%20of%20antioxidant%20compounds.pdf |
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| author | Abdullah, Sumaiyah Abdullah, Noorlidah Sabaratnam, Vikineswary Yap, Ken Choy |
| author_facet | Abdullah, Sumaiyah Abdullah, Noorlidah Sabaratnam, Vikineswary Yap, Ken Choy |
| author_sort | Abdullah, Sumaiyah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A450, 0.86) but not significantly different from LsqMeOH (A450, 0.84). FR1 and FR3 showed much lower absorbance, with values (A450, 0.21) and (A450, 0.36) respectively at 1 mg ml-1. The most active fraction (F3) was further subjected to LC-MS/MS to obtain its detailed chemical profile. Uridine, ganoderic acid derivative, and flavonoids were the first time being found in L. squarrosulus antioxidative fractions. The present results indicate that the fraction extracts of L. squarrosulus possess antioxidant properties and can be used as free radical inhibitors. Therefore, this research suggested the potentials of L. squarrosulus as a source of antioxidant extract to be used in food industries (functional food). |
| first_indexed | 2025-11-15T11:51:24Z |
| format | Article |
| id | upm-72153 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:51:24Z |
| publishDate | 2018 |
| publisher | Biotechnical Faculty, University of Ljubljana |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-721532020-03-04T08:25:57Z http://psasir.upm.edu.my/id/eprint/72153/ CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium Abdullah, Sumaiyah Abdullah, Noorlidah Sabaratnam, Vikineswary Yap, Ken Choy A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A450, 0.86) but not significantly different from LsqMeOH (A450, 0.84). FR1 and FR3 showed much lower absorbance, with values (A450, 0.21) and (A450, 0.36) respectively at 1 mg ml-1. The most active fraction (F3) was further subjected to LC-MS/MS to obtain its detailed chemical profile. Uridine, ganoderic acid derivative, and flavonoids were the first time being found in L. squarrosulus antioxidative fractions. The present results indicate that the fraction extracts of L. squarrosulus possess antioxidant properties and can be used as free radical inhibitors. Therefore, this research suggested the potentials of L. squarrosulus as a source of antioxidant extract to be used in food industries (functional food). Biotechnical Faculty, University of Ljubljana 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72153/1/CUPRAC%20assay-guided%20profiling%20of%20antioxidant%20compounds.pdf Abdullah, Sumaiyah and Abdullah, Noorlidah and Sabaratnam, Vikineswary and Yap, Ken Choy (2018) CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium. Acta Agriculturae Slovenica, 111 (1). 101 - 109. ISSN 1581-9175; ESSN: 1854-1941 http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/600 10.14720/aas.2018.111.1.10 |
| spellingShingle | Abdullah, Sumaiyah Abdullah, Noorlidah Sabaratnam, Vikineswary Yap, Ken Choy CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium |
| title | CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium |
| title_full | CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium |
| title_fullStr | CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium |
| title_full_unstemmed | CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium |
| title_short | CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium |
| title_sort | cuprac assay-guided profiling of antioxidant compounds in methanolic extract of lentinus squarrosulus mycelium |
| url | http://psasir.upm.edu.my/id/eprint/72153/ http://psasir.upm.edu.my/id/eprint/72153/ http://psasir.upm.edu.my/id/eprint/72153/ http://psasir.upm.edu.my/id/eprint/72153/1/CUPRAC%20assay-guided%20profiling%20of%20antioxidant%20compounds.pdf |