Construction and expression of synthetic gene encoding MPT64 as extracellular protein in Escherichia coli BL21 (DE3) expression system
Aim: This study was purposed to produce MPT64 protein extracellularly by contructing pelB signal peptide into expression vector containing synthetic gene of MPT64 from Mycobacterium tuberculosis H37Rv Methods: The mpt64 synthetic gene was derived from gene sequence of M. tuberculosis H37Rv. Therefor...
| Main Authors: | Kusuma, Sri Agung Fitri, Ida Parwati, Tina Rostinawati, Muhammad Yusuf, Muhammad Fadhlillah, Laily D. Tanti, Ahyudanari, Risa R., Rukayadi, Yaya, Subroto, Toto |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pharmainfo Publications
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/72109/ http://psasir.upm.edu.my/id/eprint/72109/1/Construction%20and%20expression%20of%20synthetic%20gene%20encoding%20MPT64%20as%20extracellular%20protein%20in%20Escherichia%20coli%20BL21%20%28DE3%29%20expression%20system.pdf |
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