Thermophilic Campylobacter spp. in salad vegetables in Malaysia

The main aim of this study was to combine the techniques of most probable number (MPN) and polymerase chain reaction (PCR) for quantifying the prevalence and numbers of Campylobacter spp. in ulam, a popular Malaysian salad dish, from a traditional wet market and two modern supermarkets in Selangor,...

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Main Authors: Chai, Lay Ching, Robin, Tunung, Ragavan, Usha Menon, Gunsalam, Jurin Wolmon, Abu Bakar, Fatimah, Mohamad Ghazali, Farinazleen, Radu, Son, Kumar, Malakar Pradeep
Format: Article
Language:English
Published: Elsevier 2007
Online Access:http://psasir.upm.edu.my/id/eprint/7139/
http://psasir.upm.edu.my/id/eprint/7139/1/Thermophilic%20Campylobacter%20spp.pdf
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author Chai, Lay Ching
Robin, Tunung
Ragavan, Usha Menon
Gunsalam, Jurin Wolmon
Abu Bakar, Fatimah
Mohamad Ghazali, Farinazleen
Radu, Son
Kumar, Malakar Pradeep
author_facet Chai, Lay Ching
Robin, Tunung
Ragavan, Usha Menon
Gunsalam, Jurin Wolmon
Abu Bakar, Fatimah
Mohamad Ghazali, Farinazleen
Radu, Son
Kumar, Malakar Pradeep
author_sort Chai, Lay Ching
building UPM Institutional Repository
collection Online Access
description The main aim of this study was to combine the techniques of most probable number (MPN) and polymerase chain reaction (PCR) for quantifying the prevalence and numbers of Campylobacter spp. in ulam, a popular Malaysian salad dish, from a traditional wet market and two modern supermarkets in Selangor, Malaysia. A total of 309 samples of raw vegetables which are used in ulam were examined in the study. The prevalences of campylobacters in raw vegetables were, for supermarket I, Campylobacter spp., 51.9%; Campylobacter jejuni, 40.7%; and Campylobacter coli, 35.2%: for supermarket II, Campylobacter spp., 67.7%; C. jejuni, 67.7%; and C. coli, 65.7%: and for the wet market, Campylobacter spp., 29.4%; C. jejuni, 25.5%; and C. coli, 22.6%. In addition Campylobacter fetus was detected in 1.9% of raw vegetables from supermarket I. The maximum numbers of Campylobacter spp. in raw vegetables from supermarkets and the wet market were > 2400 and 460 MPN/g, respectively.
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spelling upm-71392016-11-15T03:10:38Z http://psasir.upm.edu.my/id/eprint/7139/ Thermophilic Campylobacter spp. in salad vegetables in Malaysia Chai, Lay Ching Robin, Tunung Ragavan, Usha Menon Gunsalam, Jurin Wolmon Abu Bakar, Fatimah Mohamad Ghazali, Farinazleen Radu, Son Kumar, Malakar Pradeep The main aim of this study was to combine the techniques of most probable number (MPN) and polymerase chain reaction (PCR) for quantifying the prevalence and numbers of Campylobacter spp. in ulam, a popular Malaysian salad dish, from a traditional wet market and two modern supermarkets in Selangor, Malaysia. A total of 309 samples of raw vegetables which are used in ulam were examined in the study. The prevalences of campylobacters in raw vegetables were, for supermarket I, Campylobacter spp., 51.9%; Campylobacter jejuni, 40.7%; and Campylobacter coli, 35.2%: for supermarket II, Campylobacter spp., 67.7%; C. jejuni, 67.7%; and C. coli, 65.7%: and for the wet market, Campylobacter spp., 29.4%; C. jejuni, 25.5%; and C. coli, 22.6%. In addition Campylobacter fetus was detected in 1.9% of raw vegetables from supermarket I. The maximum numbers of Campylobacter spp. in raw vegetables from supermarkets and the wet market were > 2400 and 460 MPN/g, respectively. Elsevier 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7139/1/Thermophilic%20Campylobacter%20spp.pdf Chai, Lay Ching and Robin, Tunung and Ragavan, Usha Menon and Gunsalam, Jurin Wolmon and Abu Bakar, Fatimah and Mohamad Ghazali, Farinazleen and Radu, Son and Kumar, Malakar Pradeep (2007) Thermophilic Campylobacter spp. in salad vegetables in Malaysia. International Journal of Food Microbiology, 117 (1). pp. 106-111. ISSN 0168-1605; ESSN: 1879-3460 http://dx.doi.org/10.1016/j.ijfoodmicro.2007.02.014 10.1016/j.ijfoodmicro.2007.02.014
spellingShingle Chai, Lay Ching
Robin, Tunung
Ragavan, Usha Menon
Gunsalam, Jurin Wolmon
Abu Bakar, Fatimah
Mohamad Ghazali, Farinazleen
Radu, Son
Kumar, Malakar Pradeep
Thermophilic Campylobacter spp. in salad vegetables in Malaysia
title Thermophilic Campylobacter spp. in salad vegetables in Malaysia
title_full Thermophilic Campylobacter spp. in salad vegetables in Malaysia
title_fullStr Thermophilic Campylobacter spp. in salad vegetables in Malaysia
title_full_unstemmed Thermophilic Campylobacter spp. in salad vegetables in Malaysia
title_short Thermophilic Campylobacter spp. in salad vegetables in Malaysia
title_sort thermophilic campylobacter spp. in salad vegetables in malaysia
url http://psasir.upm.edu.my/id/eprint/7139/
http://psasir.upm.edu.my/id/eprint/7139/
http://psasir.upm.edu.my/id/eprint/7139/
http://psasir.upm.edu.my/id/eprint/7139/1/Thermophilic%20Campylobacter%20spp.pdf