Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme® IM 60 from Rhizomucor miehei, a 1,3-specific lipase, was use...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Copyright © 2005 Elsevier Ltd
2005
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/7113/ http://psasir.upm.edu.my/id/eprint/7113/1/Lipase.pdf |
| _version_ | 1848840505483329536 |
|---|---|
| author | Lai, Oi Ming Low, Chee Tein Akoh, Casimir C. |
| author_facet | Lai, Oi Ming Low, Chee Tein Akoh, Casimir C. |
| author_sort | Lai, Oi Ming |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme® IM 60 from Rhizomucor miehei, a 1,3-specific lipase, was used as the biocatalyst in this study. After 24 h of reaction, 30.5% of the total fatty acid content of the modified oil was found to be caprylic acid, indicating its incorporation into the palm olein. The triacylglycerols (TAGs) of palm olein after acidolysis were separated and were characterized by seven clusters of TAG species with equivalent carbon number (ECN), C28, C30, C32, C34, C36, C38 and C40. Caprylic–oleic–caprylic TAGs were predicted in cluster C32, which recorded the highest amount, with 35.3% of the total TAG. Fatty acid composition at the sn-2 position was determined, by pancreatic lipolysis, as C8:0, 9.2%; C12:0, 2.3%; C14:0, 1.8%; C16:0, 21.3%; C18:0, 4.7%; C18:1, 60.7%. Iodine value (IV), slip melting point (SMP) and differential scanning calorimetric (DSC) analyses of SL were also performed. In IV analysis, SL recorded a drop of value from 60.4 to 48.2 while SMP was reduced from 13 to 4.2 °C, in comparison to RBD palm olein. DSC analysis of SL gave a melting profile with two low melting peaks of −15.97 and −11.78 °C and onset temperatures of −18.43 and −14.03 °C, respectively.
|
| first_indexed | 2025-11-15T07:28:25Z |
| format | Article |
| id | upm-7113 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:28:25Z |
| publishDate | 2005 |
| publisher | Copyright © 2005 Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-71132015-10-06T00:11:08Z http://psasir.upm.edu.my/id/eprint/7113/ Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor Lai, Oi Ming Low, Chee Tein Akoh, Casimir C. Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme® IM 60 from Rhizomucor miehei, a 1,3-specific lipase, was used as the biocatalyst in this study. After 24 h of reaction, 30.5% of the total fatty acid content of the modified oil was found to be caprylic acid, indicating its incorporation into the palm olein. The triacylglycerols (TAGs) of palm olein after acidolysis were separated and were characterized by seven clusters of TAG species with equivalent carbon number (ECN), C28, C30, C32, C34, C36, C38 and C40. Caprylic–oleic–caprylic TAGs were predicted in cluster C32, which recorded the highest amount, with 35.3% of the total TAG. Fatty acid composition at the sn-2 position was determined, by pancreatic lipolysis, as C8:0, 9.2%; C12:0, 2.3%; C14:0, 1.8%; C16:0, 21.3%; C18:0, 4.7%; C18:1, 60.7%. Iodine value (IV), slip melting point (SMP) and differential scanning calorimetric (DSC) analyses of SL were also performed. In IV analysis, SL recorded a drop of value from 60.4 to 48.2 while SMP was reduced from 13 to 4.2 °C, in comparison to RBD palm olein. DSC analysis of SL gave a melting profile with two low melting peaks of −15.97 and −11.78 °C and onset temperatures of −18.43 and −14.03 °C, respectively. Copyright © 2005 Elsevier Ltd 2005-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7113/1/Lipase.pdf Lai, Oi Ming and Low, Chee Tein and Akoh, Casimir C. (2005) Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor. Food Chemistry, 92 (3). pp. 527-533. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2004.08.028 English |
| spellingShingle | Lai, Oi Ming Low, Chee Tein Akoh, Casimir C. Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor |
| title | Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
|
| title_full | Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
|
| title_fullStr | Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
|
| title_full_unstemmed | Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
|
| title_short | Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
|
| title_sort | lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor |
| url | http://psasir.upm.edu.my/id/eprint/7113/ http://psasir.upm.edu.my/id/eprint/7113/ http://psasir.upm.edu.my/id/eprint/7113/1/Lipase.pdf |