Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2005
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/7106/ http://psasir.upm.edu.my/id/eprint/7106/1/Study%20on%20response%20surface%20methodology.pdf |
| _version_ | 1848840503502569472 |
|---|---|
| author | Gunawan, Erin Ryantin Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha |
| author_facet | Gunawan, Erin Ryantin Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort | Gunawan, Erin Ryantin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (2.5–10 h), temperature (30–70 °C), amount of enzyme (0.1–0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1:1–1:5) on the percentage yield of wax esters. The optimum conditions derived via RSM were: reaction time 7.38 h, temperature 53.9 °C, amount of enzyme 0.149 g, and substrate molar ratio 1:3.41. The actual experimental yield was 84.6% under optimum condition, which compared well to the maximum predicted value of 85.4%.
|
| first_indexed | 2025-11-15T07:28:23Z |
| format | Article |
| id | upm-7106 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:28:23Z |
| publishDate | 2005 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-71062015-11-30T07:47:59Z http://psasir.upm.edu.my/id/eprint/7106/ Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester Gunawan, Erin Ryantin Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (2.5–10 h), temperature (30–70 °C), amount of enzyme (0.1–0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1:1–1:5) on the percentage yield of wax esters. The optimum conditions derived via RSM were: reaction time 7.38 h, temperature 53.9 °C, amount of enzyme 0.149 g, and substrate molar ratio 1:3.41. The actual experimental yield was 84.6% under optimum condition, which compared well to the maximum predicted value of 85.4%. Elsevier 2005-12-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7106/1/Study%20on%20response%20surface%20methodology.pdf Gunawan, Erin Ryantin and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2005) Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester. Enzyme and Microbial Technology, 37 (7). pp. 739-744. ISSN 0141-0229 http://dx.doi.org/10.1016/j.enzmictec.2005.04.010 10.1016/j.enzmictec.2005.04.010 English |
| spellingShingle | Gunawan, Erin Ryantin Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester |
| title | Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
|
| title_full | Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
|
| title_fullStr | Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
|
| title_full_unstemmed | Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
|
| title_short | Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
|
| title_sort | study on response surface methodology (rsm) of lipase-catalyzed synthesis of palm-based wax ester |
| url | http://psasir.upm.edu.my/id/eprint/7106/ http://psasir.upm.edu.my/id/eprint/7106/ http://psasir.upm.edu.my/id/eprint/7106/ http://psasir.upm.edu.my/id/eprint/7106/1/Study%20on%20response%20surface%20methodology.pdf |