Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol

Tocotrienols and tocopherols are isoforms of vitamin E. Vitamin E may exhibit antioxidant, prooxidant and non-antioxidant activities depending upon circumstances. In this study, the effect of tocotrienols and α-tocopherol on the activities of HMG CoA reductase and cholesterol 7 α-hydroxylase was inv...

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Main Authors: Teik, Khor Hun, Theng , Ng Theng, Rajendran, Raajeswari
Format: Article
Language:English
English
Published: Nutrition Society of Malaysia 2002
Online Access:http://psasir.upm.edu.my/id/eprint/7097/
http://psasir.upm.edu.my/id/eprint/7097/1/mjn8n2_art3.pdf
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author Teik, Khor Hun
Theng , Ng Theng
Rajendran, Raajeswari
author_facet Teik, Khor Hun
Theng , Ng Theng
Rajendran, Raajeswari
author_sort Teik, Khor Hun
building UPM Institutional Repository
collection Online Access
description Tocotrienols and tocopherols are isoforms of vitamin E. Vitamin E may exhibit antioxidant, prooxidant and non-antioxidant activities depending upon circumstances. In this study, the effect of tocotrienols and α-tocopherol on the activities of HMG CoA reductase and cholesterol 7 α-hydroxylase was investigated. Pure tocotrienols were isolated from palm fatty acid distillate and pure α-tocopherol was obtained commercially. Guinea pigs were treated with different dosages of tocotrienols and α-tocopherol. After the treatment period, animals were sacrificed and liver microsomes were prepared. HMG CoA reductase and cholesterol 7α-hydroxylase were assayed using tracer techniques. Our results showed that the effects of tocotrienols and α-tocopherol on the activities of both the enzymes were dose-dependent. At low dosages, both tocotrienols and α-tocopherol exhibited an inhibitory effect on both the enzymes. Moreover, tocotrienols were a much stronger inhibitors than α-tocopherol. At high dosages, on the other hand, tocotrienols and α-tocopherol showed opposite effects on the enzymes. While tocotrienols continued to exhibit an inhibitory effect, α-tocopherol actually exhibited a stimulatory effect on both the enzymes. A possible explanation for this observation is suggested.
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spelling upm-70972013-05-27T07:33:24Z http://psasir.upm.edu.my/id/eprint/7097/ Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol Teik, Khor Hun Theng , Ng Theng Rajendran, Raajeswari Tocotrienols and tocopherols are isoforms of vitamin E. Vitamin E may exhibit antioxidant, prooxidant and non-antioxidant activities depending upon circumstances. In this study, the effect of tocotrienols and α-tocopherol on the activities of HMG CoA reductase and cholesterol 7 α-hydroxylase was investigated. Pure tocotrienols were isolated from palm fatty acid distillate and pure α-tocopherol was obtained commercially. Guinea pigs were treated with different dosages of tocotrienols and α-tocopherol. After the treatment period, animals were sacrificed and liver microsomes were prepared. HMG CoA reductase and cholesterol 7α-hydroxylase were assayed using tracer techniques. Our results showed that the effects of tocotrienols and α-tocopherol on the activities of both the enzymes were dose-dependent. At low dosages, both tocotrienols and α-tocopherol exhibited an inhibitory effect on both the enzymes. Moreover, tocotrienols were a much stronger inhibitors than α-tocopherol. At high dosages, on the other hand, tocotrienols and α-tocopherol showed opposite effects on the enzymes. While tocotrienols continued to exhibit an inhibitory effect, α-tocopherol actually exhibited a stimulatory effect on both the enzymes. A possible explanation for this observation is suggested. Nutrition Society of Malaysia 2002 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7097/1/mjn8n2_art3.pdf Teik, Khor Hun and Theng , Ng Theng and Rajendran, Raajeswari (2002) Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol. Malaysian Journal of Nutrition, 8 (2). pp. 157-166. ISSN 1394-035X http://nutriweb.org.my English
spellingShingle Teik, Khor Hun
Theng , Ng Theng
Rajendran, Raajeswari
Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol
title Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol
title_full Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol
title_fullStr Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol
title_full_unstemmed Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol
title_short Dose-Dependent Cholesterolemic Activity of Tocotrienols and α-Tocopherol
title_sort dose-dependent cholesterolemic activity of tocotrienols and α-tocopherol
url http://psasir.upm.edu.my/id/eprint/7097/
http://psasir.upm.edu.my/id/eprint/7097/
http://psasir.upm.edu.my/id/eprint/7097/1/mjn8n2_art3.pdf