Nipah virus glycoprotein: production in baculovirus and application in diagnosis
A method for serological diagnosis of Nipah virus (NiV) is described. DNA encoding truncated G protein of NiV was clone into the pFastbac HT vector, and the fusion protein to His-Tag was expressed in insect cells by recombinant baculovirus. The resulting His-G recombinant fusion protein was purified...
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| Format: | Article |
| Language: | English |
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Elsevier
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/709/ http://psasir.upm.edu.my/id/eprint/709/1/Nipah%20Virus%20Glycoprotein.pdf |
| _version_ | 1848838844109029376 |
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| author | Eshaghi, Majid Tan, Wen Siang Mohd Mohidin, Taznim Begam Yusoff, Khatijah |
| author_facet | Eshaghi, Majid Tan, Wen Siang Mohd Mohidin, Taznim Begam Yusoff, Khatijah |
| author_sort | Eshaghi, Majid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A method for serological diagnosis of Nipah virus (NiV) is described. DNA encoding truncated G protein of NiV was clone into the pFastbac HT vector, and the fusion protein to His-Tag was expressed in insect cells by recombinant baculovirus. The resulting His-G recombinant fusion protein was purified by affinity chromatography and used as the coating antigen for serological testing by in direct enzyme-linked immunosorbant assay (ELISA). When tested against a panel of swine serum samples, the recombinant G protein-based ELISA successfully discriminated all 40 samples previously determined to be serum neutralizing test (SNT) positive from 11 SNT negative samples. The data show that the recombinant G protein exhibit the antogenic epitopes and conformation necessary for specific antigen-antibody recognition. The main advantage of the recombinant G protein-base NiV ELISA compared to and ELISA using whole virus antigen is the use of single antigenic protein instead of inactivated whole virus which is required to be prepared under high risk and cost. This test is suitable for routine diagnosis of NiV and also for epidemiological surveys as it allows highly reliable testing of a large number of sera rapidly. |
| first_indexed | 2025-11-15T07:02:00Z |
| format | Article |
| id | upm-709 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:02:00Z |
| publishDate | 2004 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-7092017-10-31T08:16:58Z http://psasir.upm.edu.my/id/eprint/709/ Nipah virus glycoprotein: production in baculovirus and application in diagnosis Eshaghi, Majid Tan, Wen Siang Mohd Mohidin, Taznim Begam Yusoff, Khatijah A method for serological diagnosis of Nipah virus (NiV) is described. DNA encoding truncated G protein of NiV was clone into the pFastbac HT vector, and the fusion protein to His-Tag was expressed in insect cells by recombinant baculovirus. The resulting His-G recombinant fusion protein was purified by affinity chromatography and used as the coating antigen for serological testing by in direct enzyme-linked immunosorbant assay (ELISA). When tested against a panel of swine serum samples, the recombinant G protein-based ELISA successfully discriminated all 40 samples previously determined to be serum neutralizing test (SNT) positive from 11 SNT negative samples. The data show that the recombinant G protein exhibit the antogenic epitopes and conformation necessary for specific antigen-antibody recognition. The main advantage of the recombinant G protein-base NiV ELISA compared to and ELISA using whole virus antigen is the use of single antigenic protein instead of inactivated whole virus which is required to be prepared under high risk and cost. This test is suitable for routine diagnosis of NiV and also for epidemiological surveys as it allows highly reliable testing of a large number of sera rapidly. Elsevier 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/709/1/Nipah%20Virus%20Glycoprotein.pdf Eshaghi, Majid and Tan, Wen Siang and Mohd Mohidin, Taznim Begam and Yusoff, Khatijah (2004) Nipah virus glycoprotein: production in baculovirus and application in diagnosis. Virus Research, 106 (1). pp. 71-76. ISSN 0168-1702; ESSN: 1872-7492 http://www.sciencedirect.com/science/article/pii/S0168170204002643?via%3Dihub 10.1016/j.virusres.2004.06.011 |
| spellingShingle | Eshaghi, Majid Tan, Wen Siang Mohd Mohidin, Taznim Begam Yusoff, Khatijah Nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| title | Nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| title_full | Nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| title_fullStr | Nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| title_full_unstemmed | Nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| title_short | Nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| title_sort | nipah virus glycoprotein: production in baculovirus and application in diagnosis |
| url | http://psasir.upm.edu.my/id/eprint/709/ http://psasir.upm.edu.my/id/eprint/709/ http://psasir.upm.edu.my/id/eprint/709/ http://psasir.upm.edu.my/id/eprint/709/1/Nipah%20Virus%20Glycoprotein.pdf |