Secretory expression of thermostable T1 lipase through bacteriocin release protein
The extracellular production of T1 lipase was performed by co-expression of pJL3 vector encoding bacteriocin release protein in prokaryotic system. Secretory expression was optimized by considering several parameters, including host strains, inducer (IPTG) concentration, media, induction at A600 nm,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7073/ http://psasir.upm.edu.my/id/eprint/7073/1/Secretory%20expression%20of%20thermostable%20T1%20lipase%20through%20bacteriocin%20release%20protein.pdf |
| _version_ | 1848840495217770496 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The extracellular production of T1 lipase was performed by co-expression of pJL3 vector encoding bacteriocin release protein in prokaryotic system. Secretory expression was optimized by considering several parameters, including host strains, inducer (IPTG) concentration, media, induction at A600 nm, temperature, and time of induction. Among the host strains tested, Origami B excreted out 18,100 U/ml of lipase activity into culture medium when induced with 50 μM IPTG for 12 h. The Origami B harboring recombinant plasmid pGEX/T1S and pJL3 vector was chosen for further study. IPTG at 0.05 mM, YT medium, induction at A600 nm of 1.25, 30 °C, and 32 h of induction time were best condition for T1 lipase secretion with Origami B as a host. |
| first_indexed | 2025-11-15T07:28:15Z |
| format | Article |
| id | upm-7073 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:28:15Z |
| publishDate | 2005 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-70732016-09-28T08:23:22Z http://psasir.upm.edu.my/id/eprint/7073/ Secretory expression of thermostable T1 lipase through bacteriocin release protein Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar The extracellular production of T1 lipase was performed by co-expression of pJL3 vector encoding bacteriocin release protein in prokaryotic system. Secretory expression was optimized by considering several parameters, including host strains, inducer (IPTG) concentration, media, induction at A600 nm, temperature, and time of induction. Among the host strains tested, Origami B excreted out 18,100 U/ml of lipase activity into culture medium when induced with 50 μM IPTG for 12 h. The Origami B harboring recombinant plasmid pGEX/T1S and pJL3 vector was chosen for further study. IPTG at 0.05 mM, YT medium, induction at A600 nm of 1.25, 30 °C, and 32 h of induction time were best condition for T1 lipase secretion with Origami B as a host. Elsevier 2005-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7073/1/Secretory%20expression%20of%20thermostable%20T1%20lipase%20through%20bacteriocin%20release%20protein.pdf Raja Abdul Rahman, Raja Noor Zaliha and Leow, Thean Chor and Basri, Mahiran and Salleh, Abu Bakar (2005) Secretory expression of thermostable T1 lipase through bacteriocin release protein. Protein Expression and Purification, 40 (2). pp. 411-416. ISSN 1046-5928; ESSN: 1096-0279 http://www.sciencedirect.com/science/article/pii/S1046592805000082 10.1016/j.pep.2005.01.006 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar Secretory expression of thermostable T1 lipase through bacteriocin release protein |
| title | Secretory expression of thermostable T1 lipase through bacteriocin release protein |
| title_full | Secretory expression of thermostable T1 lipase through bacteriocin release protein |
| title_fullStr | Secretory expression of thermostable T1 lipase through bacteriocin release protein |
| title_full_unstemmed | Secretory expression of thermostable T1 lipase through bacteriocin release protein |
| title_short | Secretory expression of thermostable T1 lipase through bacteriocin release protein |
| title_sort | secretory expression of thermostable t1 lipase through bacteriocin release protein |
| url | http://psasir.upm.edu.my/id/eprint/7073/ http://psasir.upm.edu.my/id/eprint/7073/ http://psasir.upm.edu.my/id/eprint/7073/ http://psasir.upm.edu.my/id/eprint/7073/1/Secretory%20expression%20of%20thermostable%20T1%20lipase%20through%20bacteriocin%20release%20protein.pdf |