An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production
An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbit...
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| Format: | Article |
| Language: | English |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7058/ http://psasir.upm.edu.my/id/eprint/7058/1/An%20organic%20solvent.pdf |
| _version_ | 1848840491274076160 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbitol as the sole carbon source, followed by starch and lactose at pH 7.0 and 37 °C. Dextrose, sucrose and glycerol greatly reduced the protease production. The best organic nitrogen source was casamino acid. Tryptone, soytone and yeast extract supported protease production while corn steep liquor and beef extract inhibited the protease activity. Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it. More than 62% drop in production occurred in the presence of amino acids. Addition of metal ions such as K+, Mg2+ and Ca2+ maximized the enzyme production. |
| first_indexed | 2025-11-15T07:28:11Z |
| format | Article |
| id | upm-7058 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:28:11Z |
| publishDate | 2005 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-70582016-09-26T08:46:39Z http://psasir.upm.edu.my/id/eprint/7058/ An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbitol as the sole carbon source, followed by starch and lactose at pH 7.0 and 37 °C. Dextrose, sucrose and glycerol greatly reduced the protease production. The best organic nitrogen source was casamino acid. Tryptone, soytone and yeast extract supported protease production while corn steep liquor and beef extract inhibited the protease activity. Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it. More than 62% drop in production occurred in the presence of amino acids. Addition of metal ions such as K+, Mg2+ and Ca2+ maximized the enzyme production. Elsevier 2005-04-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7058/1/An%20organic%20solvent.pdf Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production. Enzyme and Microbial Technology, 36 (5-6). pp. 749-757. ISSN 0141-0229; ESSN: 1879-0909 http://www.sciencedirect.com/science/article/pii/S0141022904004065 10.1016/j.enzmictec.2004.12.022 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production |
| title | An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production |
| title_full | An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production |
| title_fullStr | An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production |
| title_full_unstemmed | An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production |
| title_short | An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production |
| title_sort | organic solvent-tolerant protease from pseudomonas aeruginosa strain k: nutritional factors affecting protease production |
| url | http://psasir.upm.edu.my/id/eprint/7058/ http://psasir.upm.edu.my/id/eprint/7058/ http://psasir.upm.edu.my/id/eprint/7058/ http://psasir.upm.edu.my/id/eprint/7058/1/An%20organic%20solvent.pdf |