Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library
A random peptide library of heptamers displayed on he surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with proteinsequences of Nipah virus and several putative epitope...
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/704/ http://psasir.upm.edu.my/id/eprint/704/1/Identification%20of%20Epitopes%20In%20The%20Nucleocapsid%20Protein%20of%20Nipah%20Virus%20Using%20a%20Linear%20Phage.pdf |
| _version_ | 1848838843196768256 |
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| author | Eshaghi, Majid Wen, Siang Tan Yusoff, Khatijah |
| author_facet | Eshaghi, Majid Wen, Siang Tan Yusoff, Khatijah |
| author_sort | Eshaghi, Majid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A random peptide library of heptamers displayed on he surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with proteinsequences of Nipah virus and several putative epitopes were indentified within the nucleocapsid protein. A total of 41 to 60 (68%) selected phage clones had inserts resembling a region with the sequence SNRTQGE, located at the C-terminal end (amino acids 503-509) of the nucleocapsid protein. The binding of antibodies in the swine and human antisera to the phage clone was inhibited by synthetic peptide corresponding to this region. Epitopes identified by phage display are consistent with the predicted antigenic sites for the Nipah virus nucleocapsid protein. The selected phage clone used as a coating antigen discriminated swine and human Nipah virus sera-positive from sera-negative samples exhibit characteristics, which might be attractive for diagnostic tests. |
| first_indexed | 2025-11-15T07:01:59Z |
| format | Article |
| id | upm-704 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:01:59Z |
| publishDate | 2005 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-7042015-09-15T02:47:19Z http://psasir.upm.edu.my/id/eprint/704/ Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library Eshaghi, Majid Wen, Siang Tan Yusoff, Khatijah A random peptide library of heptamers displayed on he surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with proteinsequences of Nipah virus and several putative epitopes were indentified within the nucleocapsid protein. A total of 41 to 60 (68%) selected phage clones had inserts resembling a region with the sequence SNRTQGE, located at the C-terminal end (amino acids 503-509) of the nucleocapsid protein. The binding of antibodies in the swine and human antisera to the phage clone was inhibited by synthetic peptide corresponding to this region. Epitopes identified by phage display are consistent with the predicted antigenic sites for the Nipah virus nucleocapsid protein. The selected phage clone used as a coating antigen discriminated swine and human Nipah virus sera-positive from sera-negative samples exhibit characteristics, which might be attractive for diagnostic tests. Wiley-Blackwell 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/704/1/Identification%20of%20Epitopes%20In%20The%20Nucleocapsid%20Protein%20of%20Nipah%20Virus%20Using%20a%20Linear%20Phage.pdf Eshaghi, Majid and Wen, Siang Tan and Yusoff, Khatijah (2005) Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library. Journal of Medical Virology, 75 (1). pp. 147-152. ISSN 0146-6615 http://dx.doi.org/10.1002/jmv.20249 English |
| spellingShingle | Eshaghi, Majid Wen, Siang Tan Yusoff, Khatijah Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library |
| title | Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library |
| title_full | Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library |
| title_fullStr | Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library |
| title_full_unstemmed | Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library |
| title_short | Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library |
| title_sort | identification of epitopes in the nucleocapsid protein of nipah virus using a linear phage-displayed random peptide library |
| url | http://psasir.upm.edu.my/id/eprint/704/ http://psasir.upm.edu.my/id/eprint/704/ http://psasir.upm.edu.my/id/eprint/704/1/Identification%20of%20Epitopes%20In%20The%20Nucleocapsid%20Protein%20of%20Nipah%20Virus%20Using%20a%20Linear%20Phage.pdf |