Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
A fusion phage carrying the peptide sequence CTLTTKLYC was selected from a disulfide constrained phage display random peptide library against Newcastle disease virus (NDV) strain AF2240. The binding affinity of the phage for AF2240 is both pH and temperature-dependent with optimal binding occurring...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/702/ http://psasir.upm.edu.my/id/eprint/702/1/PFBSB4.pdf |
| _version_ | 1848838842883244032 |
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| author | Wen, Siang Tan Ramanujam, Priadarishni Nathan, Sheila Yusoff, Khatijah |
| author_facet | Wen, Siang Tan Ramanujam, Priadarishni Nathan, Sheila Yusoff, Khatijah |
| author_sort | Wen, Siang Tan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A fusion phage carrying the peptide sequence CTLTTKLYC was selected from a disulfide constrained phage display random peptide library against Newcastle disease virus (NDV) strain AF2240. The binding affinity of the phage for AF2240 is both pH and temperature-dependent with optimal binding occurring at pH 4 and 4oC respectively. No binding was observed at pH 12. Binding decreased with temperatures above 4oC until it was completely abolished at 80oC. The results suggest that the fusion activity of the virus is promoted in acidic conditions. |
| first_indexed | 2025-11-15T07:01:59Z |
| format | Article |
| id | upm-702 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:01:59Z |
| publishDate | 2004 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-7022013-05-27T06:50:15Z http://psasir.upm.edu.my/id/eprint/702/ Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus Wen, Siang Tan Ramanujam, Priadarishni Nathan, Sheila Yusoff, Khatijah A fusion phage carrying the peptide sequence CTLTTKLYC was selected from a disulfide constrained phage display random peptide library against Newcastle disease virus (NDV) strain AF2240. The binding affinity of the phage for AF2240 is both pH and temperature-dependent with optimal binding occurring at pH 4 and 4oC respectively. No binding was observed at pH 12. Binding decreased with temperatures above 4oC until it was completely abolished at 80oC. The results suggest that the fusion activity of the virus is promoted in acidic conditions. 2004 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/702/1/PFBSB4.pdf Wen, Siang Tan and Ramanujam, Priadarishni and Nathan, Sheila and Yusoff, Khatijah (2004) Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus. Malaysian Journal of Biochemistry and Molecular Biology, 9. pp. 35-37. ISSN 1511-2616 English |
| spellingShingle | Wen, Siang Tan Ramanujam, Priadarishni Nathan, Sheila Yusoff, Khatijah Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus |
| title | Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus |
| title_full | Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus |
| title_fullStr | Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus |
| title_full_unstemmed | Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus |
| title_short | Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus |
| title_sort | effect of ph and temperature on the binding of a fusion phage to newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/702/ http://psasir.upm.edu.my/id/eprint/702/1/PFBSB4.pdf |