Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats

Introduction: Thymoquinone (TQ), a bioactive compound from Nigella sativa is known for its various medicinal properties. Due to the low solubility of TQ, nanostructured lipid carrier (NLC) has been used as a delivery system to improve its efficacy. Nevertheless, the effect of TQ-NLC when administere...

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Main Authors: Saiful Yazan, Latifah, Mohd Azlan, Siti Nabilahuda, Zakarial Ansar, Fatin Hannani, Gopalsamy, Banulata
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/70044/
http://psasir.upm.edu.my/id/eprint/70044/1/2019070210012408_MJMHS_SP2_2019.pdf
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author Saiful Yazan, Latifah
Mohd Azlan, Siti Nabilahuda
Zakarial Ansar, Fatin Hannani
Gopalsamy, Banulata
author_facet Saiful Yazan, Latifah
Mohd Azlan, Siti Nabilahuda
Zakarial Ansar, Fatin Hannani
Gopalsamy, Banulata
author_sort Saiful Yazan, Latifah
building UPM Institutional Repository
collection Online Access
description Introduction: Thymoquinone (TQ), a bioactive compound from Nigella sativa is known for its various medicinal properties. Due to the low solubility of TQ, nanostructured lipid carrier (NLC) has been used as a delivery system to improve its efficacy. Nevertheless, the effect of TQ-NLC when administered intravenously is unclear. This study investigated the acute toxicity profile of intravenous administration of TQ-NLC in an in vivo model. Methods: Twelve female Sprague dawley rats were assigned randomly into two groups (n=6); a control and a treatment group that received normal saline and 25 mg/kg TQ-NLC, respectively, via intravenous injection. The rats were observed for 14 days for any alterations to their usual physical conditions such as behaviour and mortality, body weight, food intake, organ-to-body weight ratio, and haematological, biochemical and histopathological profile. Results: There were no significant changes (p>0.05) in the body weight, food intake, organ-to-body weight ratio, and haematological, bio-chemical and histopathological profile between TQ-NLC treatment and the control group. However, inflammation was observed at the site of injection on the rat’s tail. Conclusion: Intravenous administration of TQ-NLC (25 mg/kg) did not exert acute toxic effect in female Sprague dawley rats. The data can be used as a basis to further develop TQ-NLC as a potential therapeutic drug.
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spelling upm-700442019-08-16T00:38:55Z http://psasir.upm.edu.my/id/eprint/70044/ Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats Saiful Yazan, Latifah Mohd Azlan, Siti Nabilahuda Zakarial Ansar, Fatin Hannani Gopalsamy, Banulata Introduction: Thymoquinone (TQ), a bioactive compound from Nigella sativa is known for its various medicinal properties. Due to the low solubility of TQ, nanostructured lipid carrier (NLC) has been used as a delivery system to improve its efficacy. Nevertheless, the effect of TQ-NLC when administered intravenously is unclear. This study investigated the acute toxicity profile of intravenous administration of TQ-NLC in an in vivo model. Methods: Twelve female Sprague dawley rats were assigned randomly into two groups (n=6); a control and a treatment group that received normal saline and 25 mg/kg TQ-NLC, respectively, via intravenous injection. The rats were observed for 14 days for any alterations to their usual physical conditions such as behaviour and mortality, body weight, food intake, organ-to-body weight ratio, and haematological, biochemical and histopathological profile. Results: There were no significant changes (p>0.05) in the body weight, food intake, organ-to-body weight ratio, and haematological, bio-chemical and histopathological profile between TQ-NLC treatment and the control group. However, inflammation was observed at the site of injection on the rat’s tail. Conclusion: Intravenous administration of TQ-NLC (25 mg/kg) did not exert acute toxic effect in female Sprague dawley rats. The data can be used as a basis to further develop TQ-NLC as a potential therapeutic drug. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/70044/1/2019070210012408_MJMHS_SP2_2019.pdf Saiful Yazan, Latifah and Mohd Azlan, Siti Nabilahuda and Zakarial Ansar, Fatin Hannani and Gopalsamy, Banulata (2019) Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats. Malaysian Journal of Medicine and Health Sciences, 15 (SP2). pp. 51-57. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2019070210012408_MJMHS_SP2_2019.pdf
spellingShingle Saiful Yazan, Latifah
Mohd Azlan, Siti Nabilahuda
Zakarial Ansar, Fatin Hannani
Gopalsamy, Banulata
Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats
title Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats
title_full Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats
title_fullStr Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats
title_full_unstemmed Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats
title_short Acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) in Sprague dawley rats
title_sort acute toxicity study of intravenous administration of thymoquinone-loaded nanostructured lipid carrier (tq-nlc) in sprague dawley rats
url http://psasir.upm.edu.my/id/eprint/70044/
http://psasir.upm.edu.my/id/eprint/70044/
http://psasir.upm.edu.my/id/eprint/70044/1/2019070210012408_MJMHS_SP2_2019.pdf