Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop
Stevia rebaudiana, a perennial herb native to northeastern Paraguay, has gained immense attention globally over the recent decades due to the natural sweetness of its leaves. Like in most plants, this particular species contains high amount of secondary metabolites, thus rendering the isolation of h...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/69842/ http://psasir.upm.edu.my/id/eprint/69842/1/Efficient%20and%20high-quality%20RNA%20isolation%20from%20metabolite-rich%20tissues%20of%20Stevia%20rebaudiana%2C%20an%20important%20commercial%20crop.pdf |
| _version_ | 1848856522131505152 |
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| author | Tan, Yoeng Leh Yong, Christina Seok Yien Nulit, Rosimah Abdullah, Janna Ong |
| author_facet | Tan, Yoeng Leh Yong, Christina Seok Yien Nulit, Rosimah Abdullah, Janna Ong |
| author_sort | Tan, Yoeng Leh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Stevia rebaudiana, a perennial herb native to northeastern Paraguay, has gained immense attention globally over the recent decades due to the natural sweetness of its leaves. Like in most plants, this particular species contains high amount of secondary metabolites, thus rendering the isolation of high quality and quantity RNA extract for molecular applications rather challenging. An effective, high-yield and high-quality RNA isolation protocol for this economically important plant species was devised here based on the cetyltrimethylammonium bromide (CTAB) extraction method, with an additional genomic DNA (gDNA) removal step. DNA and other contaminants that may affect downstream applications were effectively removed. Our results exhibited that RNA samples isolated from the leaves and stems of Stevia rebaudiana using this improvised method are high in integrity and quality with RNA integrity number (RIN) of more than 8 and low in contaminants. |
| first_indexed | 2025-11-15T11:42:59Z |
| format | Article |
| id | upm-69842 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:42:59Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-698422019-07-08T07:26:17Z http://psasir.upm.edu.my/id/eprint/69842/ Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop Tan, Yoeng Leh Yong, Christina Seok Yien Nulit, Rosimah Abdullah, Janna Ong Stevia rebaudiana, a perennial herb native to northeastern Paraguay, has gained immense attention globally over the recent decades due to the natural sweetness of its leaves. Like in most plants, this particular species contains high amount of secondary metabolites, thus rendering the isolation of high quality and quantity RNA extract for molecular applications rather challenging. An effective, high-yield and high-quality RNA isolation protocol for this economically important plant species was devised here based on the cetyltrimethylammonium bromide (CTAB) extraction method, with an additional genomic DNA (gDNA) removal step. DNA and other contaminants that may affect downstream applications were effectively removed. Our results exhibited that RNA samples isolated from the leaves and stems of Stevia rebaudiana using this improvised method are high in integrity and quality with RNA integrity number (RIN) of more than 8 and low in contaminants. Penerbit Universiti Sains Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/69842/1/Efficient%20and%20high-quality%20RNA%20isolation%20from%20metabolite-rich%20tissues%20of%20Stevia%20rebaudiana%2C%20an%20important%20commercial%20crop.pdf Tan, Yoeng Leh and Yong, Christina Seok Yien and Nulit, Rosimah and Abdullah, Janna Ong (2019) Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop. Tropical Life Sciences Research, 30 (1). pp. 149-159. ISSN 1985-3718; ESSN: 2180-4249 http://www.tlsr.usm.my/current.html 10.21315/tlsr2019.30.1.9 |
| spellingShingle | Tan, Yoeng Leh Yong, Christina Seok Yien Nulit, Rosimah Abdullah, Janna Ong Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop |
| title | Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop |
| title_full | Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop |
| title_fullStr | Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop |
| title_full_unstemmed | Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop |
| title_short | Efficient and high-quality RNA isolation from metabolite-rich tissues of Stevia rebaudiana, an important commercial crop |
| title_sort | efficient and high-quality rna isolation from metabolite-rich tissues of stevia rebaudiana, an important commercial crop |
| url | http://psasir.upm.edu.my/id/eprint/69842/ http://psasir.upm.edu.my/id/eprint/69842/ http://psasir.upm.edu.my/id/eprint/69842/ http://psasir.upm.edu.my/id/eprint/69842/1/Efficient%20and%20high-quality%20RNA%20isolation%20from%20metabolite-rich%20tissues%20of%20Stevia%20rebaudiana%2C%20an%20important%20commercial%20crop.pdf |