Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/69286/ http://psasir.upm.edu.my/id/eprint/69286/1/Effects%20of%20N-methyl-N-nitrosourea%20on%20seed%20germination%20of%20Stevia%20rebaudiana.pdf |
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| author | Ahmad, Lyena Watty Zuraine Zainuddin, Zarina Osman, Mohamad Mohamad, Azhar Jaafar Sidik, Norrizah |
| author_facet | Ahmad, Lyena Watty Zuraine Zainuddin, Zarina Osman, Mohamad Mohamad, Azhar Jaafar Sidik, Norrizah |
| author_sort | Ahmad, Lyena Watty Zuraine |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU. |
| first_indexed | 2025-11-15T11:40:28Z |
| format | Article |
| id | upm-69286 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:40:28Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-692862020-03-25T09:03:06Z http://psasir.upm.edu.my/id/eprint/69286/ Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana Ahmad, Lyena Watty Zuraine Zainuddin, Zarina Osman, Mohamad Mohamad, Azhar Jaafar Sidik, Norrizah Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/69286/1/Effects%20of%20N-methyl-N-nitrosourea%20on%20seed%20germination%20of%20Stevia%20rebaudiana.pdf Ahmad, Lyena Watty Zuraine and Zainuddin, Zarina and Osman, Mohamad and Mohamad, Azhar and Jaafar Sidik, Norrizah (2019) Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana. Sains Malaysiana, 48 (2). pp. 301-307. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html 10.17576/jsm-2019-4802-05 |
| spellingShingle | Ahmad, Lyena Watty Zuraine Zainuddin, Zarina Osman, Mohamad Mohamad, Azhar Jaafar Sidik, Norrizah Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana |
| title | Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana |
| title_full | Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana |
| title_fullStr | Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana |
| title_full_unstemmed | Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana |
| title_short | Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana |
| title_sort | effects of n-methyl-n-nitrosourea on seed germination of stevia rebaudiana |
| url | http://psasir.upm.edu.my/id/eprint/69286/ http://psasir.upm.edu.my/id/eprint/69286/ http://psasir.upm.edu.my/id/eprint/69286/ http://psasir.upm.edu.my/id/eprint/69286/1/Effects%20of%20N-methyl-N-nitrosourea%20on%20seed%20germination%20of%20Stevia%20rebaudiana.pdf |