Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction
A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AEPress
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/692/ http://psasir.upm.edu.my/id/eprint/692/1/PFBSB8.pdf |
| _version_ | 1848838840504025088 |
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| author | Tan, Sheau Wei Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Tan, Wen Siang |
| author_facet | Tan, Sheau Wei Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Tan, Wen Siang |
| author_sort | Tan, Sheau Wei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes the melting temperature (Tm) ranged from 86°C to 87°C. The sensitivity of the real time PCR was compared with the reverse transcription (RT) – nested PCR enzyme – linked immunosorbent assay (ELISA, RT-nested PCR ELISA). Whereas the detection limit of the real time PCR was 10 pg DNA, the RT-nested PCR ELISA and conventional PCR could only detect up to 1 ng and DNA, respectively. Thus the real time PCR offers a sensitive, rapid and convenient method for screening large number of NDV specimens. |
| first_indexed | 2025-11-15T07:01:57Z |
| format | Article |
| id | upm-692 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:01:57Z |
| publishDate | 2004 |
| publisher | AEPress |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-6922018-08-20T04:38:07Z http://psasir.upm.edu.my/id/eprint/692/ Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction Tan, Sheau Wei Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Tan, Wen Siang A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes the melting temperature (Tm) ranged from 86°C to 87°C. The sensitivity of the real time PCR was compared with the reverse transcription (RT) – nested PCR enzyme – linked immunosorbent assay (ELISA, RT-nested PCR ELISA). Whereas the detection limit of the real time PCR was 10 pg DNA, the RT-nested PCR ELISA and conventional PCR could only detect up to 1 ng and DNA, respectively. Thus the real time PCR offers a sensitive, rapid and convenient method for screening large number of NDV specimens. AEPress 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/692/1/PFBSB8.pdf Tan, Sheau Wei and Omar, Abdul Rahman and Ideris, Aini and Yusoff, Khatijah and Tan, Wen Siang (2004) Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction. Acta Virologica, 48. pp. 23-28. ISSN 0001-723X; ESSN: 1336-2305 http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=45&category_id=3&option=com_virtuemart&vmcchk=1&Itemid=1 |
| spellingShingle | Tan, Sheau Wei Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Tan, Wen Siang Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction |
| title | Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction |
| title_full | Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction |
| title_fullStr | Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction |
| title_full_unstemmed | Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction |
| title_short | Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction |
| title_sort | detection of newcastle disease virus using a sybr green i real time polymerase chain reaction |
| url | http://psasir.upm.edu.my/id/eprint/692/ http://psasir.upm.edu.my/id/eprint/692/ http://psasir.upm.edu.my/id/eprint/692/1/PFBSB8.pdf |