Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells
The objectives of this study were to characterize fowl adenovirus (FAdV) isolates of Malaysia propagated in chicken embryo liver (CEL) cells. Three isolates of FAdV namely; UPM11142, UPM11134 and UPM08136 were used. They were first propagated in specific pathogen free (SPF) chicken embryonated eggs...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Faculty of Veterinary Medicine, Universiti Putra Malaysia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/67636/ http://psasir.upm.edu.my/id/eprint/67636/1/WPSA_2018-9.pdf |
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| author | Ugwu, Chidozie Clifford Bejo, Mohd Hair Mat Isa, Nurulfiza Omar, Abdul Rahman Ideris, Aini |
| author_facet | Ugwu, Chidozie Clifford Bejo, Mohd Hair Mat Isa, Nurulfiza Omar, Abdul Rahman Ideris, Aini |
| author_sort | Ugwu, Chidozie Clifford |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objectives of this study were to characterize fowl adenovirus (FAdV) isolates of Malaysia propagated in chicken embryo liver (CEL) cells. Three isolates of FAdV namely; UPM11142, UPM11134 and UPM08136 were used. They were first propagated in specific pathogen free (SPF) chicken embryonated eggs (CEE) once (E1) and further propagated in CEL cells for 5 consecutive passages (CELP1 to CELP5). The E1, CELP1 and CELP5 of all of the isolates were characterized by PCR technique. The PCR products were then sequenced and analyzed. The study showed that all the isolates caused mortality in SPF CEE eggs and adapted well in CEL cells. Eosinophilic intranuclear inclusion bodies were observed in the cells. The UPM11142 and UPM11134 exhibited more pathogenic in CEE and CEL cells than UPM08136. All of the FADV isolates were characterized as FAdV group E serotype 8b. Nucleotide and amino acid changes were recorded in CELP1 and CELP5 when compared to E1. These isolates have potential to be used for vaccine development against the disease. |
| first_indexed | 2025-11-15T11:33:10Z |
| format | Conference or Workshop Item |
| id | upm-67636 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:33:10Z |
| publishDate | 2018 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-676362019-04-09T03:47:43Z http://psasir.upm.edu.my/id/eprint/67636/ Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells Ugwu, Chidozie Clifford Bejo, Mohd Hair Mat Isa, Nurulfiza Omar, Abdul Rahman Ideris, Aini The objectives of this study were to characterize fowl adenovirus (FAdV) isolates of Malaysia propagated in chicken embryo liver (CEL) cells. Three isolates of FAdV namely; UPM11142, UPM11134 and UPM08136 were used. They were first propagated in specific pathogen free (SPF) chicken embryonated eggs (CEE) once (E1) and further propagated in CEL cells for 5 consecutive passages (CELP1 to CELP5). The E1, CELP1 and CELP5 of all of the isolates were characterized by PCR technique. The PCR products were then sequenced and analyzed. The study showed that all the isolates caused mortality in SPF CEE eggs and adapted well in CEL cells. Eosinophilic intranuclear inclusion bodies were observed in the cells. The UPM11142 and UPM11134 exhibited more pathogenic in CEE and CEL cells than UPM08136. All of the FADV isolates were characterized as FAdV group E serotype 8b. Nucleotide and amino acid changes were recorded in CELP1 and CELP5 when compared to E1. These isolates have potential to be used for vaccine development against the disease. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2018 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67636/1/WPSA_2018-9.pdf Ugwu, Chidozie Clifford and Bejo, Mohd Hair and Mat Isa, Nurulfiza and Omar, Abdul Rahman and Ideris, Aini (2018) Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells. In: 3rd World's Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2018, 18-19 Apr. 2018, Faculty of Veterinary Medicine, Universiti Putra Malaysia and Kuala Lumpur Convention Centre, Kuala Lumpur. (pp. 45-46). |
| spellingShingle | Ugwu, Chidozie Clifford Bejo, Mohd Hair Mat Isa, Nurulfiza Omar, Abdul Rahman Ideris, Aini Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells |
| title | Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells |
| title_full | Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells |
| title_fullStr | Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells |
| title_full_unstemmed | Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells |
| title_short | Characterization of three fowl adenovirus isolates of Malaysia propagated in chicken embryo liver cells |
| title_sort | characterization of three fowl adenovirus isolates of malaysia propagated in chicken embryo liver cells |
| url | http://psasir.upm.edu.my/id/eprint/67636/ http://psasir.upm.edu.my/id/eprint/67636/1/WPSA_2018-9.pdf |