Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/67186/ http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf |
| _version_ | 1848855779203874816 |
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| author | Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman |
| author_facet | Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman |
| author_sort | Khan, Muhammad Shoaib |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better understanding in the application of the non-toxic preservation technique of living tissues. The aim of this study was to evaluate the potential of antifreeze peptides (AFP) as a cryopreservative agent for living tissue e.g. skin. To determine the effects of cryopreservation on the tissue using AFP, type 1 AFP (peptide 1 m) was used to cryopreserve skin graft in different concentrations: 0.5, 1.0 and 2.0 mg/ml at -10°C or -20°C for 24 hrs. The histological distortions effects of the tissues were measured using a scoring system to determine the extent of freezing damage incurred in cryopreserved skin tissues. It was found that relatively less microscopic tissue damages occurred at -10°C compared to -20°C at higher AFP concentration. It is concluded that skin tissue cryopreservation could maintain comparatively better microscopic tissue integrity at -10°C using 2 mg/ml concentration of AFP type 1 as the cryopreservative agent. |
| first_indexed | 2025-11-15T11:31:11Z |
| format | Conference or Workshop Item |
| id | upm-67186 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:31:11Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-671862019-03-08T06:25:50Z http://psasir.upm.edu.my/id/eprint/67186/ Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better understanding in the application of the non-toxic preservation technique of living tissues. The aim of this study was to evaluate the potential of antifreeze peptides (AFP) as a cryopreservative agent for living tissue e.g. skin. To determine the effects of cryopreservation on the tissue using AFP, type 1 AFP (peptide 1 m) was used to cryopreserve skin graft in different concentrations: 0.5, 1.0 and 2.0 mg/ml at -10°C or -20°C for 24 hrs. The histological distortions effects of the tissues were measured using a scoring system to determine the extent of freezing damage incurred in cryopreserved skin tissues. It was found that relatively less microscopic tissue damages occurred at -10°C compared to -20°C at higher AFP concentration. It is concluded that skin tissue cryopreservation could maintain comparatively better microscopic tissue integrity at -10°C using 2 mg/ml concentration of AFP type 1 as the cryopreservative agent. 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf Khan, Muhammad Shoaib and Abubakar, Adamu Abdul and Abdul Rahman, Mohd Basyaruddin and Abu Bakar @ Zakaria, Md Zuki and Mohamed Mustapha, Noordin and Mohamad Yusof, Loqman (2015) Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides. In: 6th Pan Commonwealth Veterinary Conference of the CVA and 27th Veterinary Association Malaysia Congress 2015 (PCVC6 & 27VAM), 23-27 Mar. 2015, The Royale Chulan Hotel, Kuala Lumpur, Malaysia. (pp. 473-476). |
| spellingShingle | Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| title | Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| title_full | Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| title_fullStr | Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| title_full_unstemmed | Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| title_short | Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| title_sort | histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
| url | http://psasir.upm.edu.my/id/eprint/67186/ http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf |