Hepatic cytometry in postmortem interval estimation in dogs
Estimation of postmortem interval (PMI) is a prerequisite in forensic invesitgations of death in all species of interest to courts. Age-old methods of relying on gross postmortem changes including algor mortis, livor mortis, putrefaction and lividity are been replaced by rapid high end techniques su...
| Main Authors: | , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/67181/ http://psasir.upm.edu.my/id/eprint/67181/1/6th%20PCVC%20AND%2027th%20VAM-10.pdf |
| _version_ | 1848855777831288832 |
|---|---|
| author | Okene, Ibrahim Abdul-Azeez Mohamed Mustapha, Noordin Abu Daud, Nur Hardy Shaari, Rumaizi |
| author_facet | Okene, Ibrahim Abdul-Azeez Mohamed Mustapha, Noordin Abu Daud, Nur Hardy Shaari, Rumaizi |
| author_sort | Okene, Ibrahim Abdul-Azeez |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Estimation of postmortem interval (PMI) is a prerequisite in forensic invesitgations of death in all species of interest to courts. Age-old methods of relying on gross postmortem changes including algor mortis, livor mortis, putrefaction and lividity are been replaced by rapid high end techniques such as gene expression changes, proteomics, electrical impulse alterations, histopathological and biochemical changes. Generally cell death – autolysis or necrosis begins as soon as nutrition and oxygen supply to cells are cut short. On this basis that techniques such as flow cytometry have the potential of becoming a handy tool in assessing the viability of cells and tissues at postmortem while correlating same with postmortem interval. This preliminary study aimed at correlating hepatic tissue necrosis with postmortem interval in dogs, sampled the hepatic tissue of three (n=3) bitches at 6 h intrevals to assess the rate of necrosis over an 18 h period. Propidium iodide stain was used to filter dead from living cells on flow cyometry. Results were analyzed by descriptive statistics in percentages and coefficents and summarily showed a strongly linear correlation (R2=0.98) between postmortem hepatic tissue autolysis and postmortem interval in dogs. Rate of hepatic tissue autolysis measured by flow cytometry is a good indicator of postmortem interval in dogs. |
| first_indexed | 2025-11-15T11:31:10Z |
| format | Conference or Workshop Item |
| id | upm-67181 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:31:10Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-671812019-03-08T06:25:37Z http://psasir.upm.edu.my/id/eprint/67181/ Hepatic cytometry in postmortem interval estimation in dogs Okene, Ibrahim Abdul-Azeez Mohamed Mustapha, Noordin Abu Daud, Nur Hardy Shaari, Rumaizi Estimation of postmortem interval (PMI) is a prerequisite in forensic invesitgations of death in all species of interest to courts. Age-old methods of relying on gross postmortem changes including algor mortis, livor mortis, putrefaction and lividity are been replaced by rapid high end techniques such as gene expression changes, proteomics, electrical impulse alterations, histopathological and biochemical changes. Generally cell death – autolysis or necrosis begins as soon as nutrition and oxygen supply to cells are cut short. On this basis that techniques such as flow cytometry have the potential of becoming a handy tool in assessing the viability of cells and tissues at postmortem while correlating same with postmortem interval. This preliminary study aimed at correlating hepatic tissue necrosis with postmortem interval in dogs, sampled the hepatic tissue of three (n=3) bitches at 6 h intrevals to assess the rate of necrosis over an 18 h period. Propidium iodide stain was used to filter dead from living cells on flow cyometry. Results were analyzed by descriptive statistics in percentages and coefficents and summarily showed a strongly linear correlation (R2=0.98) between postmortem hepatic tissue autolysis and postmortem interval in dogs. Rate of hepatic tissue autolysis measured by flow cytometry is a good indicator of postmortem interval in dogs. 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67181/1/6th%20PCVC%20AND%2027th%20VAM-10.pdf Okene, Ibrahim Abdul-Azeez and Mohamed Mustapha, Noordin and Abu Daud, Nur Hardy and Shaari, Rumaizi (2015) Hepatic cytometry in postmortem interval estimation in dogs. In: 6th Pan Commonwealth Veterinary Conference of the CVA and 27th Veterinary Association Malaysia Congress 2015 (PCVC6 & 27VAM), 23-27 Mar. 2015, The Royale Chulan Hotel, Kuala Lumpur, Malaysia. (pp. 428-431). |
| spellingShingle | Okene, Ibrahim Abdul-Azeez Mohamed Mustapha, Noordin Abu Daud, Nur Hardy Shaari, Rumaizi Hepatic cytometry in postmortem interval estimation in dogs |
| title | Hepatic cytometry in postmortem interval estimation in dogs |
| title_full | Hepatic cytometry in postmortem interval estimation in dogs |
| title_fullStr | Hepatic cytometry in postmortem interval estimation in dogs |
| title_full_unstemmed | Hepatic cytometry in postmortem interval estimation in dogs |
| title_short | Hepatic cytometry in postmortem interval estimation in dogs |
| title_sort | hepatic cytometry in postmortem interval estimation in dogs |
| url | http://psasir.upm.edu.my/id/eprint/67181/ http://psasir.upm.edu.my/id/eprint/67181/1/6th%20PCVC%20AND%2027th%20VAM-10.pdf |