Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia

Isolation and biochemical characterization of diazotrophs were carried out on seven soil series/types of Tanjong Karang Rice Irrigation Project area. The soil population ranged from 4 × 104 to 2.2 × 106 cfu g-1 soil. Diazotrophic populations were significantly (P<0.01) influenced by soil types, p...

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Main Authors: Naher, Umme Aminun, Othman, Radziah, Shamsuddin, Zulkifli, Mohd Saud, Halimi, Ismail, Mohd Razi
Format: Article
Language:English
English
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/6671/
http://psasir.upm.edu.my/id/eprint/6671/1/Isolation%20of%20Diazotrophs%20from%20Different%20Soils%20.pdf
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author Naher, Umme Aminun
Othman, Radziah
Shamsuddin, Zulkifli
Mohd Saud, Halimi
Ismail, Mohd Razi
author_facet Naher, Umme Aminun
Othman, Radziah
Shamsuddin, Zulkifli
Mohd Saud, Halimi
Ismail, Mohd Razi
author_sort Naher, Umme Aminun
building UPM Institutional Repository
collection Online Access
description Isolation and biochemical characterization of diazotrophs were carried out on seven soil series/types of Tanjong Karang Rice Irrigation Project area. The soil population ranged from 4 × 104 to 2.2 × 106 cfu g-1 soil. Diazotrophic populations were significantly (P<0.01) influenced by soil types, plant age and rice varieties. Higher soil and rhizosphere populations were recorded in Organic Clay and Muck, Bakau, Sedu and Serong soils. The highest root (6.3 × 107) and shoot (2.5 × 107) populations were found in MR219 rice planted in Organic Clay and Muck and Sedu soil series, which had higher C, N and P contents. The highest acetylene reduction assay (ARA) value (1.26 × 10-6 μmoL C2H4 cfu-1 hr1) was found in isolate Sb35. Several diazotrophic strains produced 32 to 69 mg L-1 of indoleacetic acid(IAA). The highest IAA was produced by the diazotrophic strain Sb41 (Corynebacterium sp). Eleven of the diazotrophic strains isolated from root and shoot of the rice varieties were capable of producing cellulose degrading enzyme. Tanjong Karang rice growing area harbor a diverse group of bacteria and most of the isolates belonged to the genera of Rhizobium, Burkholderia and Corynebacterium.
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institution Universiti Putra Malaysia
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spelling upm-66712015-12-18T07:32:28Z http://psasir.upm.edu.my/id/eprint/6671/ Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia Naher, Umme Aminun Othman, Radziah Shamsuddin, Zulkifli Mohd Saud, Halimi Ismail, Mohd Razi Isolation and biochemical characterization of diazotrophs were carried out on seven soil series/types of Tanjong Karang Rice Irrigation Project area. The soil population ranged from 4 × 104 to 2.2 × 106 cfu g-1 soil. Diazotrophic populations were significantly (P<0.01) influenced by soil types, plant age and rice varieties. Higher soil and rhizosphere populations were recorded in Organic Clay and Muck, Bakau, Sedu and Serong soils. The highest root (6.3 × 107) and shoot (2.5 × 107) populations were found in MR219 rice planted in Organic Clay and Muck and Sedu soil series, which had higher C, N and P contents. The highest acetylene reduction assay (ARA) value (1.26 × 10-6 μmoL C2H4 cfu-1 hr1) was found in isolate Sb35. Several diazotrophic strains produced 32 to 69 mg L-1 of indoleacetic acid(IAA). The highest IAA was produced by the diazotrophic strain Sb41 (Corynebacterium sp). Eleven of the diazotrophic strains isolated from root and shoot of the rice varieties were capable of producing cellulose degrading enzyme. Tanjong Karang rice growing area harbor a diverse group of bacteria and most of the isolates belonged to the genera of Rhizobium, Burkholderia and Corynebacterium. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6671/1/Isolation%20of%20Diazotrophs%20from%20Different%20Soils%20.pdf Naher, Umme Aminun and Othman, Radziah and Shamsuddin, Zulkifli and Mohd Saud, Halimi and Ismail, Mohd Razi (2009) Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia. International Journal of Agriculture and Biology, 11 (5). pp. 547-552. ISSN 1560-8530 http://www.fspublishers.org/ijab/past-issues/IJABVOL_11_NO_5/8.pdf English
spellingShingle Naher, Umme Aminun
Othman, Radziah
Shamsuddin, Zulkifli
Mohd Saud, Halimi
Ismail, Mohd Razi
Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia
title Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia
title_full Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia
title_fullStr Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia
title_full_unstemmed Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia
title_short Isolation of Diazotrophs from Different Soils of Tanjong Karang Rice Growing Area in Malaysia
title_sort isolation of diazotrophs from different soils of tanjong karang rice growing area in malaysia
url http://psasir.upm.edu.my/id/eprint/6671/
http://psasir.upm.edu.my/id/eprint/6671/
http://psasir.upm.edu.my/id/eprint/6671/1/Isolation%20of%20Diazotrophs%20from%20Different%20Soils%20.pdf