Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. ma...
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| Format: | Article |
| Language: | English |
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SCIENCEDOMAIN International
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65352/ http://psasir.upm.edu.my/id/eprint/65352/1/Investigation%20of%20phytochemical%20components%20and%20bioautography%20of%20Garcinia%20mangostana%20L.%20methanol%20leaf%20extract.pdf |
| _version_ | 1848855259631321088 |
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| author | Alsultan, Qamar Mohammed Naji Sijam, Kamaruzaman Rashid, Tavga Sulaiman Ahmad, Khairulmazmi Awla, Hayman Kakakhan |
| author_facet | Alsultan, Qamar Mohammed Naji Sijam, Kamaruzaman Rashid, Tavga Sulaiman Ahmad, Khairulmazmi Awla, Hayman Kakakhan |
| author_sort | Alsultan, Qamar Mohammed Naji |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. mangostana rich in alkaloids, Flavonoids, Saponins, Tannins, Phenol, Terpenoids, Anthraquinone and Cardiac glycosides. A simple bioautographic procedure, involving spraying suspensions of the bacteria on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial compounds present in the extract. This activity was indicated by white spots against a red background on the TLC plates after spraying with 5% TTC aqueous solution. P. syringae and X. oryzae were inhibited by the most compounds separated on the TLC plates from the extract. |
| first_indexed | 2025-11-15T11:22:55Z |
| format | Article |
| id | upm-65352 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:22:55Z |
| publishDate | 2017 |
| publisher | SCIENCEDOMAIN International |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-653522018-10-08T02:25:24Z http://psasir.upm.edu.my/id/eprint/65352/ Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract Alsultan, Qamar Mohammed Naji Sijam, Kamaruzaman Rashid, Tavga Sulaiman Ahmad, Khairulmazmi Awla, Hayman Kakakhan The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. mangostana rich in alkaloids, Flavonoids, Saponins, Tannins, Phenol, Terpenoids, Anthraquinone and Cardiac glycosides. A simple bioautographic procedure, involving spraying suspensions of the bacteria on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial compounds present in the extract. This activity was indicated by white spots against a red background on the TLC plates after spraying with 5% TTC aqueous solution. P. syringae and X. oryzae were inhibited by the most compounds separated on the TLC plates from the extract. SCIENCEDOMAIN International 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65352/1/Investigation%20of%20phytochemical%20components%20and%20bioautography%20of%20Garcinia%20mangostana%20L.%20methanol%20leaf%20extract.pdf Alsultan, Qamar Mohammed Naji and Sijam, Kamaruzaman and Rashid, Tavga Sulaiman and Ahmad, Khairulmazmi and Awla, Hayman Kakakhan (2017) Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract. Journal of Experimental Agriculture International, 15 (3). art. no. JEAI.31398. pp. 1-7. ISSN 2457-0591 http://www.sciencedomain.org/abstract/17732 10.9734/JEAI/2017/31398 |
| spellingShingle | Alsultan, Qamar Mohammed Naji Sijam, Kamaruzaman Rashid, Tavga Sulaiman Ahmad, Khairulmazmi Awla, Hayman Kakakhan Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract |
| title | Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract |
| title_full | Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract |
| title_fullStr | Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract |
| title_full_unstemmed | Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract |
| title_short | Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract |
| title_sort | investigation of phytochemical components and bioautography of garcinia mangostana l. methanol leaf extract |
| url | http://psasir.upm.edu.my/id/eprint/65352/ http://psasir.upm.edu.my/id/eprint/65352/ http://psasir.upm.edu.my/id/eprint/65352/ http://psasir.upm.edu.my/id/eprint/65352/1/Investigation%20of%20phytochemical%20components%20and%20bioautography%20of%20Garcinia%20mangostana%20L.%20methanol%20leaf%20extract.pdf |