Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment

Resistant starch is the non-digestible portion of starch that reaches the colon and act as a prebiotic to stimulate the activity and growth of beneficial gut microbiota. In the present study, resistant starch content of native (lemantak), commercialized and retrograded sago and starch was analysed,...

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Main Authors: Arshad, N. H., Mohd Zaman, Siti Aisyah, Rawi, M. H., Sarbini, Shahrul Razid
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/65320/
http://psasir.upm.edu.my/id/eprint/65320/1/%2810%29.pdf
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author Arshad, N. H.
Mohd Zaman, Siti Aisyah
Rawi, M. H.
Sarbini, Shahrul Razid
author_facet Arshad, N. H.
Mohd Zaman, Siti Aisyah
Rawi, M. H.
Sarbini, Shahrul Razid
author_sort Arshad, N. H.
building UPM Institutional Repository
collection Online Access
description Resistant starch is the non-digestible portion of starch that reaches the colon and act as a prebiotic to stimulate the activity and growth of beneficial gut microbiota. In the present study, resistant starch content of native (lemantak), commercialized and retrograded sago and starch was analysed, and the in vitro fermentability with known probiotics were investigated. Retrograded starch was produced through two cycles of autoclaving and cooling steps. The resistant starch content of each modified starch were measured based on the method approved by AOAC 2002.02. The in vitro batch fermentation was carried out with inoculation of Lactobacillus acidophilus and Bifidobacterium animalis at 37°C for 24 hours in anaerobic condition. Total bacteria was enumerated at 0, 6, 12 and 24 hours. Highest resistant starch content was shown in lemantak (native sago starch) at 62.61%. Lemantak was also shown to be the most preferred fermentation substrate with the highest number of total bacterial count at all sampling hours. These findings suggest the potential of lemantak as a prebiotic.
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spelling upm-653202018-10-05T07:30:23Z http://psasir.upm.edu.my/id/eprint/65320/ Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment Arshad, N. H. Mohd Zaman, Siti Aisyah Rawi, M. H. Sarbini, Shahrul Razid Resistant starch is the non-digestible portion of starch that reaches the colon and act as a prebiotic to stimulate the activity and growth of beneficial gut microbiota. In the present study, resistant starch content of native (lemantak), commercialized and retrograded sago and starch was analysed, and the in vitro fermentability with known probiotics were investigated. Retrograded starch was produced through two cycles of autoclaving and cooling steps. The resistant starch content of each modified starch were measured based on the method approved by AOAC 2002.02. The in vitro batch fermentation was carried out with inoculation of Lactobacillus acidophilus and Bifidobacterium animalis at 37°C for 24 hours in anaerobic condition. Total bacteria was enumerated at 0, 6, 12 and 24 hours. Highest resistant starch content was shown in lemantak (native sago starch) at 62.61%. Lemantak was also shown to be the most preferred fermentation substrate with the highest number of total bacterial count at all sampling hours. These findings suggest the potential of lemantak as a prebiotic. Faculty of Food Science and Technology, Universiti Putra Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65320/1/%2810%29.pdf Arshad, N. H. and Mohd Zaman, Siti Aisyah and Rawi, M. H. and Sarbini, Shahrul Razid (2018) Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment. International Food Research Journal, 25 (3). pp. 951-957. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/25%20(03)%202018/(10).pdf
spellingShingle Arshad, N. H.
Mohd Zaman, Siti Aisyah
Rawi, M. H.
Sarbini, Shahrul Razid
Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
title Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
title_full Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
title_fullStr Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
title_full_unstemmed Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
title_short Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
title_sort resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment
url http://psasir.upm.edu.my/id/eprint/65320/
http://psasir.upm.edu.my/id/eprint/65320/
http://psasir.upm.edu.my/id/eprint/65320/1/%2810%29.pdf