Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology

Partial hydrolysis using Lipozyme RMIM lipase in a solvent-free system was used to produce a diacylglycerol (DAG)-enriched palm olein. Response surface methodology (RSM) was applied to model and optimize the reaction conditions namely water content (30– 70 wt% of enzyme mass), enzyme load (5–15 wt...

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Main Authors:	Cheong, Ling Zhi, Tan, Chin Ping, Arifin, Norlelawati, Lo, Seong Koon, Lai, Oi Ming
Format:	Article
Language:	English English
Published:	Elsevier 2007
Online Access:	http://psasir.upm.edu.my/id/eprint/6516/ http://psasir.upm.edu.my/id/eprint/6516/1/Production%20of%20a%20diacylglycerol.pdf

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author	Cheong, Ling Zhi Tan, Chin Ping Arifin, Norlelawati Lo, Seong Koon Lai, Oi Ming
author_facet	Cheong, Ling Zhi Tan, Chin Ping Arifin, Norlelawati Lo, Seong Koon Lai, Oi Ming
author_sort	Cheong, Ling Zhi
building	UPM Institutional Repository
collection	Online Access
description	Partial hydrolysis using Lipozyme RMIM lipase in a solvent-free system was used to produce a diacylglycerol (DAG)-enriched palm olein. Response surface methodology (RSM) was applied to model and optimize the reaction conditions namely water content (30– 70 wt% of enzyme mass), enzyme load (5–15 wt% of oil mass), reaction temperature (45–85 C) and reaction time (6–16 h). Well fitting models were successfully established for both DAG yield (R2 = 0.8788) and unhydrolysed triacylglycerol (TAG) (R2 = 0.8653) through multiple linear regressions with backward elimination. Chi-square test indicated that there were no significant (P > 0.05) differences between the observed and predicted values for both models. All reaction conditions had positive effects on DAG yield and negative effects on unhydrolysed TAG. Optimal reaction conditions were: 50 wt% water content, 10 wt% enzyme load, 65C of reaction temperature and 12 h of reaction time. The process was further up-scaled to a 9 kg production in a continuous packed bed bioreactor. Results indicated that upscaling was possible with a similar DAG yield (32 wt%) as in lab scale. Purification of the DAG oil using short path distillation yielded a DAG-enriched palm olein with 60 wt% DAG and 40 wt% TAG which is suitable for margarine, spread or shortening applications.
first_indexed	2025-11-15T07:26:44Z
format	Article
id	upm-6516
institution	Universiti Putra Malaysia
institution_category	Local University
language	English English
last_indexed	2025-11-15T07:26:44Z
publishDate	2007
publisher	Elsevier
recordtype	eprints
repository_type	Digital Repository
spelling	upm-65162015-11-27T01:42:27Z http://psasir.upm.edu.my/id/eprint/6516/ Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology Cheong, Ling Zhi Tan, Chin Ping Arifin, Norlelawati Lo, Seong Koon Lai, Oi Ming Partial hydrolysis using Lipozyme RMIM lipase in a solvent-free system was used to produce a diacylglycerol (DAG)-enriched palm olein. Response surface methodology (RSM) was applied to model and optimize the reaction conditions namely water content (30– 70 wt% of enzyme mass), enzyme load (5–15 wt% of oil mass), reaction temperature (45–85 C) and reaction time (6–16 h). Well fitting models were successfully established for both DAG yield (R2 = 0.8788) and unhydrolysed triacylglycerol (TAG) (R2 = 0.8653) through multiple linear regressions with backward elimination. Chi-square test indicated that there were no significant (P > 0.05) differences between the observed and predicted values for both models. All reaction conditions had positive effects on DAG yield and negative effects on unhydrolysed TAG. Optimal reaction conditions were: 50 wt% water content, 10 wt% enzyme load, 65C of reaction temperature and 12 h of reaction time. The process was further up-scaled to a 9 kg production in a continuous packed bed bioreactor. Results indicated that upscaling was possible with a similar DAG yield (32 wt%) as in lab scale. Purification of the DAG oil using short path distillation yielded a DAG-enriched palm olein with 60 wt% DAG and 40 wt% TAG which is suitable for margarine, spread or shortening applications. Elsevier 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6516/1/Production%20of%20a%20diacylglycerol.pdf Cheong, Ling Zhi and Tan, Chin Ping and Arifin, Norlelawati and Lo, Seong Koon and Lai, Oi Ming (2007) Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology. Food Chemistry, 105 (4). pp. 1614-1622. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2007.03.070 10.1016/j.foodchem.2007.03.070 English
spellingShingle	Cheong, Ling Zhi Tan, Chin Ping Arifin, Norlelawati Lo, Seong Koon Lai, Oi Ming Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
title	Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
title_full	Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
title_fullStr	Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
title_full_unstemmed	Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
title_short	Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
title_sort	production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
url	http://psasir.upm.edu.my/id/eprint/6516/ http://psasir.upm.edu.my/id/eprint/6516/ http://psasir.upm.edu.my/id/eprint/6516/ http://psasir.upm.edu.my/id/eprint/6516/1/Production%20of%20a%20diacylglycerol.pdf

Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology

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