Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis : optimization using response surface methodology
Partial hydrolysis using Lipozyme RMIM lipase in a solvent-free system was used to produce a diacylglycerol (DAG)-enriched palm olein. Response surface methodology (RSM) was applied to model and optimize the reaction conditions namely water content (30– 70 wt% of enzyme mass), enzyme load (5–15 wt...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6516/ http://psasir.upm.edu.my/id/eprint/6516/1/Production%20of%20a%20diacylglycerol.pdf |
| Summary: | Partial hydrolysis using Lipozyme RMIM lipase in a solvent-free system was used to produce a diacylglycerol (DAG)-enriched palm
olein. Response surface methodology (RSM) was applied to model and optimize the reaction conditions namely water content (30–
70 wt% of enzyme mass), enzyme load (5–15 wt% of oil mass), reaction temperature (45–85 C) and reaction time (6–16 h). Well fitting
models were successfully established for both DAG yield (R2 = 0.8788) and unhydrolysed triacylglycerol (TAG) (R2 = 0.8653) through
multiple linear regressions with backward elimination. Chi-square test indicated that there were no significant (P > 0.05) differences
between the observed and predicted values for both models. All reaction conditions had positive effects on DAG yield and negative
effects on unhydrolysed TAG. Optimal reaction conditions were: 50 wt% water content, 10 wt% enzyme load, 65C of reaction temperature
and 12 h of reaction time. The process was further up-scaled to a 9 kg production in a continuous packed bed bioreactor. Results
indicated that upscaling was possible with a similar DAG yield (32 wt%) as in lab scale. Purification of the DAG oil using short path
distillation yielded a DAG-enriched palm olein with 60 wt% DAG and 40 wt% TAG which is suitable for margarine, spread or shortening
applications. |
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