Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus
Fowlpox is a viral disease of chicken caused by fowlpox virus (FWPV). Compared to other viral infectious diseases, studies on the interaction of antigen-presenting cells (APC) such as dendritic cells (DCs) and macrophage with FWPV remains limited. Therefore, this work was aimed to characterize cytok...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Faculty of Veterinary Medicine, Universiti Putra Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65042/ http://psasir.upm.edu.my/id/eprint/65042/1/PA-41.pdf |
| _version_ | 1848855175068909568 |
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| author | Yusof, Sakinah Abd. Rahaman, Nor Yasmin Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah |
| author_facet | Yusof, Sakinah Abd. Rahaman, Nor Yasmin Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah |
| author_sort | Yusof, Sakinah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fowlpox is a viral disease of chicken caused by fowlpox virus (FWPV). Compared to other viral infectious diseases, studies on the interaction of antigen-presenting cells (APC) such as dendritic cells (DCs) and macrophage with FWPV remains limited. Therefore, this work was aimed to characterize cytokine responses upon infection of recombinant fowlpox viruses (rFWPV) expressing H5 gene of avian influenza virus (AIV) compared to wild type FWPV on chicken DCs and macrophage cells. Chicken bone marrow cells were first isolated. The cells were differentiated into DCs (GM-CSF and IL-4 acted as supplements in RPMI media), or into macrophages (no supplements added). In order to quantify chicken IL-15 and IL-18 cytokine expression, qPCR assays based on real-time analysis were performed. Data suggested that rFWPV induced higher expression for IL-15 and IL-18 on macrophage while wild type FWPV induced higher expression for IL-15 and IL-18 on DC. |
| first_indexed | 2025-11-15T11:21:35Z |
| format | Conference or Workshop Item |
| id | upm-65042 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:21:35Z |
| publishDate | 2015 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-650422018-09-03T04:49:35Z http://psasir.upm.edu.my/id/eprint/65042/ Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus Yusof, Sakinah Abd. Rahaman, Nor Yasmin Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah Fowlpox is a viral disease of chicken caused by fowlpox virus (FWPV). Compared to other viral infectious diseases, studies on the interaction of antigen-presenting cells (APC) such as dendritic cells (DCs) and macrophage with FWPV remains limited. Therefore, this work was aimed to characterize cytokine responses upon infection of recombinant fowlpox viruses (rFWPV) expressing H5 gene of avian influenza virus (AIV) compared to wild type FWPV on chicken DCs and macrophage cells. Chicken bone marrow cells were first isolated. The cells were differentiated into DCs (GM-CSF and IL-4 acted as supplements in RPMI media), or into macrophages (no supplements added). In order to quantify chicken IL-15 and IL-18 cytokine expression, qPCR assays based on real-time analysis were performed. Data suggested that rFWPV induced higher expression for IL-15 and IL-18 on macrophage while wild type FWPV induced higher expression for IL-15 and IL-18 on DC. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65042/1/PA-41.pdf Yusof, Sakinah and Abd. Rahaman, Nor Yasmin and Omar, Abdul Rahman and Abdul Razak, Mariatulqabtiah (2015) Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus. In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 197-199). |
| spellingShingle | Yusof, Sakinah Abd. Rahaman, Nor Yasmin Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| title | Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| title_full | Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| title_fullStr | Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| title_full_unstemmed | Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| title_short | Comparison of expression for IL-15 and IL-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| title_sort | comparison of expression for il-15 and il-18 on dendritic cells and macrophages upon infection with recombinant fowlpox virus versus wild type fowlpox virus |
| url | http://psasir.upm.edu.my/id/eprint/65042/ http://psasir.upm.edu.my/id/eprint/65042/1/PA-41.pdf |