Determination of Daidzein and Genistein Contents in Mangifera Fruit.
The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely ‘bacang’ (Mangifera foetida), ‘kuini’ (M. odorata) and ‘bambangan’ (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Nutrition Society of Malaysia
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6495/ http://psasir.upm.edu.my/id/eprint/6495/1/mjn14n2_art6.pdf |
| _version_ | 1848840393607610368 |
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| author | Khoo, Hock Eng Ahmad, Ismail |
| author_facet | Khoo, Hock Eng Ahmad, Ismail |
| author_sort | Khoo, Hock Eng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of this study was to determine the daidzein and genistein contents in
Mangifera fruits. Three Mangifera species namely ‘bacang’ (Mangifera foetida), ‘kuini’
(M. odorata) and ‘bambangan’ (M. pajang) each from two different locations were
selected. The extraction of isoflavones was carried out at 80oC for 30, 60 and 90
min. HPLC method was performed with a flow rate of 1.00 ml/min using three
different separation columns to determine isoflavone contents. The Zorbax
Eclipse RP C18 reverse-phase column was found to give the best resolution for
isoflavone separation in Mangifera fruits. Moreover, extraction time of 90 min
was found to increase the isoflavone aglycone contents. At optimised condition,
‘kuini’ had relatively high daidzein (9.4-10.5 mg/100 g) and genistein (1.6-1.7
mg/100 g) contents. Daidzein content of ‘bambangan’ (8.3-8.7 mg/100 g) was
higher than ‘bacang’, but the genistein content of ‘bambangan’ (0.4-0.6 mg/100
g) was similar to that of ‘bacang’ (0.4-0.8 mg/100 g). However, there was a
variation in daidzein and genistein contents in Mangifera fruits between two
geographical locations. |
| first_indexed | 2025-11-15T07:26:38Z |
| format | Article |
| id | upm-6495 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:26:38Z |
| publishDate | 2008 |
| publisher | Nutrition Society of Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-64952014-09-18T08:54:09Z http://psasir.upm.edu.my/id/eprint/6495/ Determination of Daidzein and Genistein Contents in Mangifera Fruit. Khoo, Hock Eng Ahmad, Ismail The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely ‘bacang’ (Mangifera foetida), ‘kuini’ (M. odorata) and ‘bambangan’ (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out at 80oC for 30, 60 and 90 min. HPLC method was performed with a flow rate of 1.00 ml/min using three different separation columns to determine isoflavone contents. The Zorbax Eclipse RP C18 reverse-phase column was found to give the best resolution for isoflavone separation in Mangifera fruits. Moreover, extraction time of 90 min was found to increase the isoflavone aglycone contents. At optimised condition, ‘kuini’ had relatively high daidzein (9.4-10.5 mg/100 g) and genistein (1.6-1.7 mg/100 g) contents. Daidzein content of ‘bambangan’ (8.3-8.7 mg/100 g) was higher than ‘bacang’, but the genistein content of ‘bambangan’ (0.4-0.6 mg/100 g) was similar to that of ‘bacang’ (0.4-0.8 mg/100 g). However, there was a variation in daidzein and genistein contents in Mangifera fruits between two geographical locations. Nutrition Society of Malaysia 2008-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6495/1/mjn14n2_art6.pdf Khoo, Hock Eng and Ahmad, Ismail (2008) Determination of Daidzein and Genistein Contents in Mangifera Fruit. Malaysian Journal of Nutrition, 14 (2). pp. 189-198. ISSN 1394-035X http://nutriweb.org.my/publications/mjn0014_2/mjn14n2_art6.php English |
| spellingShingle | Khoo, Hock Eng Ahmad, Ismail Determination of Daidzein and Genistein Contents in Mangifera Fruit. |
| title | Determination of Daidzein and Genistein Contents in
Mangifera Fruit. |
| title_full | Determination of Daidzein and Genistein Contents in
Mangifera Fruit. |
| title_fullStr | Determination of Daidzein and Genistein Contents in
Mangifera Fruit. |
| title_full_unstemmed | Determination of Daidzein and Genistein Contents in
Mangifera Fruit. |
| title_short | Determination of Daidzein and Genistein Contents in
Mangifera Fruit. |
| title_sort | determination of daidzein and genistein contents in
mangifera fruit. |
| url | http://psasir.upm.edu.my/id/eprint/6495/ http://psasir.upm.edu.my/id/eprint/6495/ http://psasir.upm.edu.my/id/eprint/6495/1/mjn14n2_art6.pdf |