Biological Properties of Tinospora crispa (Akar Patawali) and Its Antiproliferative Activities on Selected Human Cancer Cell Lines
The antioxidant and anti-proliferative activity of the aqueous crude extract of Tinospora crispa stem was investigated. The proximate composition of its stem and leaves was determined. Proximate analysis revealed that T. crispa contains - protein: leaves = 4.7%, stem = 1.2%; fat: leaves = 1.5%, s...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Nutrition Society of Malaysia
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6493/ http://psasir.upm.edu.my/id/eprint/6493/1/mjn14n2_art2.pdf |
| Summary: | The antioxidant and anti-proliferative activity of the aqueous crude extract of
Tinospora crispa stem was investigated. The proximate composition of its stem
and leaves was determined. Proximate analysis revealed that T. crispa contains -
protein: leaves = 4.7%, stem = 1.2%; fat: leaves = 1.5%, stem = 0.43%; carbohydrate:
leaves = 11.8%, stem = 19.4%; ash: leaves = 2.7%, stem = 1.1%; moisture: leaves =
79.3%, stem = 77.9%; fibre: leaves = 1.59%, stem = 0.65%; and energy: leaves =
1.59%, stem = 0.65%. The antioxidant activity of the extract prepared at various
temperatures and incubation time was evaluated to determine the optimum
extraction procedure. Based on DPPH and TBA tests, the preparation of the
extract at 60oC for 6 hours was established as the best possible method as it
demonstrated the highest inhibition percentage. The extract was tested against
brine shrimp to evaluate its toxicity and no significant toxicity was recorded
since the IC50 value was more than 1000 μg/ml. The extract produced moderate
anti-proliferative activity on selected human cancer cell lines (IC50 MCF-7: 107
μg/ml, HeLa: 165 μg/ml, Caov-3: 100 μg/ml, and HepG2: 165 μg/ml). The
findings from this study suggest that T. crispa has the potential to be a source of
natural antioxidants and nutrients, besides having a moderate anti-proliferative
effect on selected human cancer cell lines.
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