Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach
Rice is one of the most important staple foods for human. However, millions hectares of rice land in the South and Southeast Asia were left uncultivated and/or grown with very low yields due to salinity. Due to this, development and production of salt-tolerant rice seed as apart of integrated manage...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64319/ http://psasir.upm.edu.my/id/eprint/64319/1/BIO%20Oral%20111117%201.pdf |
| _version_ | 1848854967996121088 |
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| author | Nulit, Rosimah Kalhori, Nahid Zulkifly, Shahrizim |
| author_facet | Nulit, Rosimah Kalhori, Nahid Zulkifly, Shahrizim |
| author_sort | Nulit, Rosimah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Rice is one of the most important staple foods for human. However, millions hectares of rice land in the South and Southeast Asia were left uncultivated and/or grown with very low yields due to salinity. Due to this, development and production of salt-tolerant rice seed as apart of integrated management practices is needed. By using tissue culture approach, salt-tolerant lines of Malaysia indica rice cv. MR219 were produced. This work started with production and propagation of MR219 callus. Then, callus was sub-cultured separately on MS media supplemented with 2 mg/L 2,4-D and different concentration of NaCl (0, 50, 100, 200, and 300 mM NaCl) to produce salt-tolerant MR219 callus. Screening and selection of salt-tolerant MR219 callus were conducted using morphological and biochemical markers which are total proline content, total soluble sugar, lipid peroxidase and the activity of ascorbate peroxidase and catalase. At regeneration of salt-tolerant plantlets, selected salt-tolerant callus was sub-cultured on MS media supplemented with 2 mg/L kinetin and 1 mg/L BAP for shoot induction, followed by sub-cultured in MS media supplemented with 0.5 mg/L BAP, 1 mg/L kinetin, 1 mg/L IBA and 0.5 mg/L NAA for root formation. At acclimatization stage, MR219 plantlets from 50 mM NaCl found survived and transferred to pots containing paddy soil. These plantlets is called First generation (F1) salt-tolerant MR219. After 70 days, seeds of F1-salt-tolerant MR219 lines was successfully obtained. The grain characteristics of mother plant and F1-salt-tolerant MR219 lines were compared. Germination capability of F1-salt-tolerant MR219 seed in saline showed that seeds of F1-salt-tolerant MR219 able to germinate and growth in 50 -100 mM NaCl. As conclusion, salt-tolerant MR219 rice was produced in vitro and have potential to be commercialized. The protocol to produce salt-tolerant rice can be used to produce other salt-tolerant plant. |
| first_indexed | 2025-11-15T11:18:17Z |
| format | Conference or Workshop Item |
| id | upm-64319 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:18:17Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-643192018-07-04T02:36:01Z http://psasir.upm.edu.my/id/eprint/64319/ Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach Nulit, Rosimah Kalhori, Nahid Zulkifly, Shahrizim Rice is one of the most important staple foods for human. However, millions hectares of rice land in the South and Southeast Asia were left uncultivated and/or grown with very low yields due to salinity. Due to this, development and production of salt-tolerant rice seed as apart of integrated management practices is needed. By using tissue culture approach, salt-tolerant lines of Malaysia indica rice cv. MR219 were produced. This work started with production and propagation of MR219 callus. Then, callus was sub-cultured separately on MS media supplemented with 2 mg/L 2,4-D and different concentration of NaCl (0, 50, 100, 200, and 300 mM NaCl) to produce salt-tolerant MR219 callus. Screening and selection of salt-tolerant MR219 callus were conducted using morphological and biochemical markers which are total proline content, total soluble sugar, lipid peroxidase and the activity of ascorbate peroxidase and catalase. At regeneration of salt-tolerant plantlets, selected salt-tolerant callus was sub-cultured on MS media supplemented with 2 mg/L kinetin and 1 mg/L BAP for shoot induction, followed by sub-cultured in MS media supplemented with 0.5 mg/L BAP, 1 mg/L kinetin, 1 mg/L IBA and 0.5 mg/L NAA for root formation. At acclimatization stage, MR219 plantlets from 50 mM NaCl found survived and transferred to pots containing paddy soil. These plantlets is called First generation (F1) salt-tolerant MR219. After 70 days, seeds of F1-salt-tolerant MR219 lines was successfully obtained. The grain characteristics of mother plant and F1-salt-tolerant MR219 lines were compared. Germination capability of F1-salt-tolerant MR219 seed in saline showed that seeds of F1-salt-tolerant MR219 able to germinate and growth in 50 -100 mM NaCl. As conclusion, salt-tolerant MR219 rice was produced in vitro and have potential to be commercialized. The protocol to produce salt-tolerant rice can be used to produce other salt-tolerant plant. 2017 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64319/1/BIO%20Oral%20111117%201.pdf Nulit, Rosimah and Kalhori, Nahid and Zulkifly, Shahrizim (2017) Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach. In: 5th International Symposium on Applied Engineering and Sciences (SAES2017), 14-15 Nov. 2017, Universiti Putra Malaysia. (p. 1). |
| spellingShingle | Nulit, Rosimah Kalhori, Nahid Zulkifly, Shahrizim Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach |
| title | Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach |
| title_full | Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach |
| title_fullStr | Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach |
| title_full_unstemmed | Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach |
| title_short | Development of salt-tolerant lines of Malaysian indica rice (Oryza sativa L. cv. MR219) using tissue culture approach |
| title_sort | development of salt-tolerant lines of malaysian indica rice (oryza sativa l. cv. mr219) using tissue culture approach |
| url | http://psasir.upm.edu.my/id/eprint/64319/ http://psasir.upm.edu.my/id/eprint/64319/1/BIO%20Oral%20111117%201.pdf |