In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1
Development of an efficient regeneration system for commercial tomato cultivars is a necessary precondition for genetic manipulation. Regeneration system of tomato variety MT1 was developed. Seeds were cultured on half strength Murashig and Skoog (MS) medium. The 12-14 days old cotyledon leaf explan...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Sarawak Biodiversity Centre
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64279/ http://psasir.upm.edu.my/id/eprint/64279/1/PROCEEDING_100719-16.pdf |
| _version_ | 1848854957238779904 |
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| author | Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran |
| author_facet | Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran |
| author_sort | Kamaladini, Hossein |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Development of an efficient regeneration system for commercial tomato cultivars is a necessary precondition for genetic manipulation. Regeneration system of tomato variety MT1 was developed. Seeds were cultured on half strength Murashig and Skoog (MS) medium. The 12-14 days old cotyledon leaf explants were cut and cultured on full strength MS medium supplemented with 1 mg/l NAA and 1 mg/l BAP. After 48 h these explants were transferred to regeneration medium containing two types of cytokinin which were 2ip and zeatin. The concentrations used were 0, 1, 2, 3, 4, 5 mg/l for both 2ip and zeatin. High frequency of shoot regeneration by using different concentrations of 2ip was achieved on MS medium with 4 mg/l giving the best results. Meanwhile MS medium containing 2 mg/l zeatin showed highest percentage and number of shoots formed per explant. Results showed that more callus formation occurred on medium containing 2ip. Individual shoots derived from cotyledon were used for root induction. Root induction medium was MS containing 5 mg/l IAA and shoots maintained on this media for three weeks until roots were well developed. These systems would provide the means for recovering genetically modified plants after subjecting explants to Agrobacterium-mediated transformation or particle bombardment. |
| first_indexed | 2025-11-15T11:18:07Z |
| format | Conference or Workshop Item |
| id | upm-64279 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:18:07Z |
| publishDate | 2008 |
| publisher | Sarawak Biodiversity Centre |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-642792018-07-04T02:31:34Z http://psasir.upm.edu.my/id/eprint/64279/ In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Development of an efficient regeneration system for commercial tomato cultivars is a necessary precondition for genetic manipulation. Regeneration system of tomato variety MT1 was developed. Seeds were cultured on half strength Murashig and Skoog (MS) medium. The 12-14 days old cotyledon leaf explants were cut and cultured on full strength MS medium supplemented with 1 mg/l NAA and 1 mg/l BAP. After 48 h these explants were transferred to regeneration medium containing two types of cytokinin which were 2ip and zeatin. The concentrations used were 0, 1, 2, 3, 4, 5 mg/l for both 2ip and zeatin. High frequency of shoot regeneration by using different concentrations of 2ip was achieved on MS medium with 4 mg/l giving the best results. Meanwhile MS medium containing 2 mg/l zeatin showed highest percentage and number of shoots formed per explant. Results showed that more callus formation occurred on medium containing 2ip. Individual shoots derived from cotyledon were used for root induction. Root induction medium was MS containing 5 mg/l IAA and shoots maintained on this media for three weeks until roots were well developed. These systems would provide the means for recovering genetically modified plants after subjecting explants to Agrobacterium-mediated transformation or particle bombardment. Sarawak Biodiversity Centre 2008 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64279/1/PROCEEDING_100719-16.pdf Kamaladini, Hossein and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran (2008) In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 450-453). |
| spellingShingle | Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 |
| title | In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 |
| title_full | In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 |
| title_fullStr | In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 |
| title_full_unstemmed | In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 |
| title_short | In vitro shoot regeneration from cotyledonary leaf explant of tomato variety MT1 |
| title_sort | in vitro shoot regeneration from cotyledonary leaf explant of tomato variety mt1 |
| url | http://psasir.upm.edu.my/id/eprint/64279/ http://psasir.upm.edu.my/id/eprint/64279/1/PROCEEDING_100719-16.pdf |