Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry

An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and...

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Main Authors: Haddadi, Fatemeh, Abdul Aziz, Maheran, Saleh, Ghizan, Abdul Rashid, Azmi, Kamaladini, Hossein
Format: Conference or Workshop Item
Language:English
Published: Sarawak Biodiversity Centre 2008
Online Access:http://psasir.upm.edu.my/id/eprint/64278/
http://psasir.upm.edu.my/id/eprint/64278/1/PROCEEDING_100719-15.pdf
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author Haddadi, Fatemeh
Abdul Aziz, Maheran
Saleh, Ghizan
Abdul Rashid, Azmi
Kamaladini, Hossein
author_facet Haddadi, Fatemeh
Abdul Aziz, Maheran
Saleh, Ghizan
Abdul Rashid, Azmi
Kamaladini, Hossein
author_sort Haddadi, Fatemeh
building UPM Institutional Repository
collection Online Access
description An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and 0 to 27 μM BAP. MS medium supplemented with a moderate concentration of 4 μM BAP in combination with 2 μM TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins, a 100 % shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation.
first_indexed 2025-11-15T11:18:07Z
format Conference or Workshop Item
id upm-64278
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T11:18:07Z
publishDate 2008
publisher Sarawak Biodiversity Centre
recordtype eprints
repository_type Digital Repository
spelling upm-642782018-07-04T02:31:36Z http://psasir.upm.edu.my/id/eprint/64278/ Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry Haddadi, Fatemeh Abdul Aziz, Maheran Saleh, Ghizan Abdul Rashid, Azmi Kamaladini, Hossein An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and 0 to 27 μM BAP. MS medium supplemented with a moderate concentration of 4 μM BAP in combination with 2 μM TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins, a 100 % shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation. Sarawak Biodiversity Centre 2008 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64278/1/PROCEEDING_100719-15.pdf Haddadi, Fatemeh and Abdul Aziz, Maheran and Saleh, Ghizan and Abdul Rashid, Azmi and Kamaladini, Hossein (2008) Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 443-446).
spellingShingle Haddadi, Fatemeh
Abdul Aziz, Maheran
Saleh, Ghizan
Abdul Rashid, Azmi
Kamaladini, Hossein
Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_full Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_fullStr Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_full_unstemmed Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_short Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry
title_sort effect of tdz and bap on shoot proliferation from in vitro shoot tips of strawberry
url http://psasir.upm.edu.my/id/eprint/64278/
http://psasir.upm.edu.my/id/eprint/64278/1/PROCEEDING_100719-15.pdf