Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay
In this work, a temporal monitoring work for heavy metals from an effluent discharge point in the Juru Industrial Estate was carried out using the protease extracted from garlic (Allium sativum) as the principal bioassay system. Casein-Coomassie-dye binding assay method has utilized this purpose. Th...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64026/ http://psasir.upm.edu.my/id/eprint/64026/1/Near%20real-time%20biomonitoring%20of%20copper%20from%20an%20industrial%20complex%20effluent%20discharge%20site%20using%20a%20plant%20protease%20inhibitive%20assay.pdf |
| _version_ | 1848854888829681664 |
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| author | Halmi, Mohd Izuan Effendi Khayat, Mohd Ezuan Gunasekaran, Baskaran Masdor, Noor Azlina Abdul Rahman, Mohd Fadhil |
| author_facet | Halmi, Mohd Izuan Effendi Khayat, Mohd Ezuan Gunasekaran, Baskaran Masdor, Noor Azlina Abdul Rahman, Mohd Fadhil |
| author_sort | Halmi, Mohd Izuan Effendi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this work, a temporal monitoring work for heavy metals from an effluent discharge point in the Juru Industrial Estate was carried out using the protease extracted from garlic (Allium sativum) as the principal bioassay system. Casein-Coomassie-dye binding assay method has utilized this purpose. The periodic sampling results for one day of a location in the Juru Industrial Estate showed temporal variation of copper concentration coinciding with garlic protease inhibition with the highest concentrations of copper occurring between 12.00 and 16.00 hours of between 3 and 3.5 mg/L copper. The crude proteases extracted from Allium sativum successfully detect temporal variation of copper form this location. In conclusion, this assay method has the potential to be a rapid, sensitive, and economic inhibitive assay for the large-scale biomonitoring works for the heavy metal copper from this area. |
| first_indexed | 2025-11-15T11:17:02Z |
| format | Article |
| id | upm-64026 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:17:02Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-640262018-06-08T00:51:46Z http://psasir.upm.edu.my/id/eprint/64026/ Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay Halmi, Mohd Izuan Effendi Khayat, Mohd Ezuan Gunasekaran, Baskaran Masdor, Noor Azlina Abdul Rahman, Mohd Fadhil In this work, a temporal monitoring work for heavy metals from an effluent discharge point in the Juru Industrial Estate was carried out using the protease extracted from garlic (Allium sativum) as the principal bioassay system. Casein-Coomassie-dye binding assay method has utilized this purpose. The periodic sampling results for one day of a location in the Juru Industrial Estate showed temporal variation of copper concentration coinciding with garlic protease inhibition with the highest concentrations of copper occurring between 12.00 and 16.00 hours of between 3 and 3.5 mg/L copper. The crude proteases extracted from Allium sativum successfully detect temporal variation of copper form this location. In conclusion, this assay method has the potential to be a rapid, sensitive, and economic inhibitive assay for the large-scale biomonitoring works for the heavy metal copper from this area. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64026/1/Near%20real-time%20biomonitoring%20of%20copper%20from%20an%20industrial%20complex%20effluent%20discharge%20site%20using%20a%20plant%20protease%20inhibitive%20assay.pdf Halmi, Mohd Izuan Effendi and Khayat, Mohd Ezuan and Gunasekaran, Baskaran and Masdor, Noor Azlina and Abdul Rahman, Mohd Fadhil (2016) Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay. Bioremediation Science and Technology Research, 4 (1). pp. 10-13. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/365 |
| spellingShingle | Halmi, Mohd Izuan Effendi Khayat, Mohd Ezuan Gunasekaran, Baskaran Masdor, Noor Azlina Abdul Rahman, Mohd Fadhil Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| title | Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| title_full | Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| title_fullStr | Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| title_full_unstemmed | Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| title_short | Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| title_sort | near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay |
| url | http://psasir.upm.edu.my/id/eprint/64026/ http://psasir.upm.edu.my/id/eprint/64026/ http://psasir.upm.edu.my/id/eprint/64026/1/Near%20real-time%20biomonitoring%20of%20copper%20from%20an%20industrial%20complex%20effluent%20discharge%20site%20using%20a%20plant%20protease%20inhibitive%20assay.pdf |