Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium
2,4-dinitrophenol (2,4-DNP) is utilized in the production of wood preservatives, dyes, and also as a pesticide. Human acute (short-term) exposure to 2,4-DNP in humans by means of oral exposure are nausea or vomiting, sweating, headaches, dizziness, and weight reduction. Thus, the removal of this com...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64024/ http://psasir.upm.edu.my/id/eprint/64024/1/Isolation%20and%20characterization%20of%20a%202%2C4-dinitrophenol-degrading%20bacterium.pdf |
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| author | Md. Zin, Noor Suffiah Padrilah, Siti Nadzirah Abdul Rahman, Mohd Fadhil Sim, Han Koh Khalid, Ariff Abd. Shukor, Mohd. Yunus |
| author_facet | Md. Zin, Noor Suffiah Padrilah, Siti Nadzirah Abdul Rahman, Mohd Fadhil Sim, Han Koh Khalid, Ariff Abd. Shukor, Mohd. Yunus |
| author_sort | Md. Zin, Noor Suffiah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | 2,4-dinitrophenol (2,4-DNP) is utilized in the production of wood preservatives, dyes, and also as a pesticide. Human acute (short-term) exposure to 2,4-DNP in humans by means of oral exposure are nausea or vomiting, sweating, headaches, dizziness, and weight reduction. Thus, the removal of this compound is highly sought. A 2,4-DNP-degrading bacterium (isolate 1) was isolated from a sample soil from Terengganu. This bacterium (isolate 1) was characterized as a rod Gram positive, non-sporulated, and non-motile bacterium. The bacterium is oxidase negative and had catalase positive activity and was able to grow aerobically on 2,4-dinitrophenol as the sole carbon source. This bacterium showed maximal growth on 2,4-DNP at the temperature optimum of 30 °C, pH 5.0 and was tolerant to 2,4-DNP concentration of up to 0.5 mM (0.092 g/L). This bacterium prefers to use urea as the nitrogen source in addition to yeast extract for mineral source and vitamin precursors. |
| first_indexed | 2025-11-15T11:17:01Z |
| format | Article |
| id | upm-64024 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:17:01Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-640242018-06-08T00:51:50Z http://psasir.upm.edu.my/id/eprint/64024/ Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium Md. Zin, Noor Suffiah Padrilah, Siti Nadzirah Abdul Rahman, Mohd Fadhil Sim, Han Koh Khalid, Ariff Abd. Shukor, Mohd. Yunus 2,4-dinitrophenol (2,4-DNP) is utilized in the production of wood preservatives, dyes, and also as a pesticide. Human acute (short-term) exposure to 2,4-DNP in humans by means of oral exposure are nausea or vomiting, sweating, headaches, dizziness, and weight reduction. Thus, the removal of this compound is highly sought. A 2,4-DNP-degrading bacterium (isolate 1) was isolated from a sample soil from Terengganu. This bacterium (isolate 1) was characterized as a rod Gram positive, non-sporulated, and non-motile bacterium. The bacterium is oxidase negative and had catalase positive activity and was able to grow aerobically on 2,4-dinitrophenol as the sole carbon source. This bacterium showed maximal growth on 2,4-DNP at the temperature optimum of 30 °C, pH 5.0 and was tolerant to 2,4-DNP concentration of up to 0.5 mM (0.092 g/L). This bacterium prefers to use urea as the nitrogen source in addition to yeast extract for mineral source and vitamin precursors. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64024/1/Isolation%20and%20characterization%20of%20a%202%2C4-dinitrophenol-degrading%20bacterium.pdf Md. Zin, Noor Suffiah and Padrilah, Siti Nadzirah and Abdul Rahman, Mohd Fadhil and Sim, Han Koh and Khalid, Ariff and Abd. Shukor, Mohd. Yunus (2016) Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium. Bioremediation Science and Technology Research, 4 (2). pp. 28-33. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/373 |
| spellingShingle | Md. Zin, Noor Suffiah Padrilah, Siti Nadzirah Abdul Rahman, Mohd Fadhil Sim, Han Koh Khalid, Ariff Abd. Shukor, Mohd. Yunus Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| title | Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| title_full | Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| title_fullStr | Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| title_full_unstemmed | Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| title_short | Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| title_sort | isolation and characterization of a 2,4-dinitrophenol-degrading bacterium |
| url | http://psasir.upm.edu.my/id/eprint/64024/ http://psasir.upm.edu.my/id/eprint/64024/ http://psasir.upm.edu.my/id/eprint/64024/1/Isolation%20and%20characterization%20of%20a%202%2C4-dinitrophenol-degrading%20bacterium.pdf |