Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection
In this work we assess the potential of acetylcholinesterase (AChE) from Oreochromis mossambicus (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from Oreochromis mossambicus brain tissues using affinity chromatography gel...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64021/ http://psasir.upm.edu.my/id/eprint/64021/1/Assessment%20of%20acetylcholinesterase%20%28AChE%29%20from%20Oreochromis%20mossambicus%20%28Cuvier%2C%201831%29%20as%20a%20source%20of%20enzyme%20for%20insecticides%20detection.pdf |
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| author | Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus |
| author_facet | Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus |
| author_sort | Muhammad, Umar Abubakar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this work we assess the potential of acetylcholinesterase (AChE) from Oreochromis mossambicus (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from Oreochromis mossambicus brain tissues using affinity chromatography gel (procainamide–Sephacryl S-1000) showed that the partially purified AChE was most active on acetylthiocholine (ATC) but had low activities on propionylthiocholine (PTC) and butyrylthiocholine (BTC), indicating that the partially purified fraction was predominantly AChE. Soluble AChE was partially purified 9.27-fold with a 91.12% yield. The partially purified AChE displayed the highest activity on ATC at pH 7 and at 30oC using 0.1 M Tris buffer. The enzyme exhibited Michaelis-Menten kinetic constants, Km, for ATC, BTC and PTC at 36, 77 and 250 µM, respectively, and the maximum velocities, Vmax, were 18.75, 0.12 and 0.05 µmol/min/mg protein, respectively. Moreover, the AChE from Oreochromis mossambicus presented comparable sensitivity to carbamates and organophosphates insecticides than that from Electrophorus electricus and many other fish AChE by comparing half maximal inhibitory concentration values. Therefore, the enzyme is a valuable source for insecticides detection in Malaysian waters at lower cost. |
| first_indexed | 2025-11-15T11:17:00Z |
| format | Article |
| id | upm-64021 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:17:00Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-640212018-06-08T00:51:55Z http://psasir.upm.edu.my/id/eprint/64021/ Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus In this work we assess the potential of acetylcholinesterase (AChE) from Oreochromis mossambicus (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from Oreochromis mossambicus brain tissues using affinity chromatography gel (procainamide–Sephacryl S-1000) showed that the partially purified AChE was most active on acetylthiocholine (ATC) but had low activities on propionylthiocholine (PTC) and butyrylthiocholine (BTC), indicating that the partially purified fraction was predominantly AChE. Soluble AChE was partially purified 9.27-fold with a 91.12% yield. The partially purified AChE displayed the highest activity on ATC at pH 7 and at 30oC using 0.1 M Tris buffer. The enzyme exhibited Michaelis-Menten kinetic constants, Km, for ATC, BTC and PTC at 36, 77 and 250 µM, respectively, and the maximum velocities, Vmax, were 18.75, 0.12 and 0.05 µmol/min/mg protein, respectively. Moreover, the AChE from Oreochromis mossambicus presented comparable sensitivity to carbamates and organophosphates insecticides than that from Electrophorus electricus and many other fish AChE by comparing half maximal inhibitory concentration values. Therefore, the enzyme is a valuable source for insecticides detection in Malaysian waters at lower cost. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64021/1/Assessment%20of%20acetylcholinesterase%20%28AChE%29%20from%20Oreochromis%20mossambicus%20%28Cuvier%2C%201831%29%20as%20a%20source%20of%20enzyme%20for%20insecticides%20detection.pdf Muhammad, Umar Abubakar and Tham, Lik Gin and Perumal, Natarajan and Mohd Daud, Hassan and Yasid, Nur Adeela and Abd. Shukor, Mohd. Yunus (2016) Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection. Bioremediation Science and Technology Research, 4 (2). pp. 11-17. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/370 |
| spellingShingle | Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection |
| title | Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection |
| title_full | Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection |
| title_fullStr | Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection |
| title_full_unstemmed | Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection |
| title_short | Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection |
| title_sort | assessment of acetylcholinesterase (ache) from oreochromis mossambicus (cuvier, 1831) as a source of enzyme for insecticides detection |
| url | http://psasir.upm.edu.my/id/eprint/64021/ http://psasir.upm.edu.my/id/eprint/64021/ http://psasir.upm.edu.my/id/eprint/64021/1/Assessment%20of%20acetylcholinesterase%20%28AChE%29%20from%20Oreochromis%20mossambicus%20%28Cuvier%2C%201831%29%20as%20a%20source%20of%20enzyme%20for%20insecticides%20detection.pdf |