Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth
Isolate JR1 was isolated from the polluted textile industry activities site in the Juru Penang area. This bacterium was characterized as a gram-positive Bacillus bacterium and also gave a positive biochemical test for catalase test and oxidase test. The isolate JR1 gave a maximum decolourization of...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64005/ http://psasir.upm.edu.my/id/eprint/64005/1/Isolation%20and%20culture%20medium%20optimisation%20using%20one-factor-at-time%20and%20response%20surface%20methodology%20on%20the%20biodegradation%20of%20the%20azo-dye%20amaranth.pdf |
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| author | Islahuddin, Noris Kartika Sari Halmi, Mohd Izuan Effendi Manogaran, Motharasan Abd. Shukor, Mohd. Yunus |
| author_facet | Islahuddin, Noris Kartika Sari Halmi, Mohd Izuan Effendi Manogaran, Motharasan Abd. Shukor, Mohd. Yunus |
| author_sort | Islahuddin, Noris Kartika Sari |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Isolate JR1 was isolated from the polluted textile industry activities site in the Juru Penang area. This bacterium was characterized as a gram-positive Bacillus bacterium and also gave a positive biochemical test for catalase test and oxidase test. The isolate JR1 gave a maximum decolourization of Amaranth dye under static conditions with the rate of decolorization of 98.82%. Seven variables which are pH, temperature (°C), ammonium acetate (g/L), glucose (g/L), sodium chloride (g/L), yeast (g/L) and dye concentration (ppm) was run by using Plackett-Burman design for the effective parameter of the decolourization of Amaranth. From the seven variables, three effective variables which were ammonium acetate, glucose, and dye concentration were further optimized by using a central composite design. The optimum value of ammonium acetate concentration at 0.74 g/L, glucose concentration at 3.0 g/L and a dye concentration at 58.1 ppm gave the highest percentage of decolourization. Thus, this isolate could provide an alternate solution in removing toxic dyes from environments. |
| first_indexed | 2025-11-15T11:16:57Z |
| format | Article |
| id | upm-64005 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:16:57Z |
| publishDate | 2017 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-640052018-06-08T00:45:02Z http://psasir.upm.edu.my/id/eprint/64005/ Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth Islahuddin, Noris Kartika Sari Halmi, Mohd Izuan Effendi Manogaran, Motharasan Abd. Shukor, Mohd. Yunus Isolate JR1 was isolated from the polluted textile industry activities site in the Juru Penang area. This bacterium was characterized as a gram-positive Bacillus bacterium and also gave a positive biochemical test for catalase test and oxidase test. The isolate JR1 gave a maximum decolourization of Amaranth dye under static conditions with the rate of decolorization of 98.82%. Seven variables which are pH, temperature (°C), ammonium acetate (g/L), glucose (g/L), sodium chloride (g/L), yeast (g/L) and dye concentration (ppm) was run by using Plackett-Burman design for the effective parameter of the decolourization of Amaranth. From the seven variables, three effective variables which were ammonium acetate, glucose, and dye concentration were further optimized by using a central composite design. The optimum value of ammonium acetate concentration at 0.74 g/L, glucose concentration at 3.0 g/L and a dye concentration at 58.1 ppm gave the highest percentage of decolourization. Thus, this isolate could provide an alternate solution in removing toxic dyes from environments. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64005/1/Isolation%20and%20culture%20medium%20optimisation%20using%20one-factor-at-time%20and%20response%20surface%20methodology%20on%20the%20biodegradation%20of%20the%20azo-dye%20amaranth.pdf Islahuddin, Noris Kartika Sari and Halmi, Mohd Izuan Effendi and Manogaran, Motharasan and Abd. Shukor, Mohd. Yunus (2017) Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth. Bioremediation Science and Technology Research, 5 (2). pp. 25-31. ISSN 2289-5892 http://journal.hibiscuspublisher.com/index.php/BSTR/article/view/360 |
| spellingShingle | Islahuddin, Noris Kartika Sari Halmi, Mohd Izuan Effendi Manogaran, Motharasan Abd. Shukor, Mohd. Yunus Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| title | Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| title_full | Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| title_fullStr | Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| title_full_unstemmed | Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| title_short | Isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| title_sort | isolation and culture medium optimisation using one-factor-at-time and response surface methodology on the biodegradation of the azo-dye amaranth |
| url | http://psasir.upm.edu.my/id/eprint/64005/ http://psasir.upm.edu.my/id/eprint/64005/ http://psasir.upm.edu.my/id/eprint/64005/1/Isolation%20and%20culture%20medium%20optimisation%20using%20one-factor-at-time%20and%20response%20surface%20methodology%20on%20the%20biodegradation%20of%20the%20azo-dye%20amaranth.pdf |