DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR
The origin of gelatin used in gelatin capsule has always been doubtful as it is not always stated in the labelling. Thus, the halal status of the gelatin is questionable and it becomes a great concern by the Muslims. Gelatin is mainly produced from the skin and bones of pig and cow which undergoes e...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Halal Products Research Institute, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63957/ http://psasir.upm.edu.my/id/eprint/63957/1/M38.pdf |
| _version_ | 1848854869766569984 |
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| author | Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi |
| author_facet | Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi |
| author_sort | Mohamad, Nurhidayatul Asma |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The origin of gelatin used in gelatin capsule has always been doubtful as it is not always stated in the labelling. Thus, the halal status of the gelatin is questionable and it becomes a great concern by the Muslims. Gelatin is mainly produced from the skin and bones of pig and cow which undergoes either acid or alkali process. Due to the extreme processing conditions, the DNA may encounter high level of degradation. Further processing of the gelatin into gelatin capsule would make it worst. Hence, the minute amount of DNA contained in the gelain and gelatin capsule require careful extraction method and sensitive detection. In this work, the extraction method is optimized in terms of DNA concentration, DNA fragmentation and the presence of real-time PCR inhibitor. Following quality assessment of the DNA extracted, both bovine and porcine are detected using species-specific real-time PCR systems based on SYBR Green reaction.The quality of the DNA extracted from gelatin and gelatin capsules are low in terms of DNA concentration. Using real-time PCR, up to approximately 300 bp target sequence can be amplified and successful detection of both species has been achieved. |
| first_indexed | 2025-11-15T11:16:43Z |
| format | Conference or Workshop Item |
| id | upm-63957 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:16:43Z |
| publishDate | 2014 |
| publisher | Halal Products Research Institute, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-639572018-06-08T00:27:37Z http://psasir.upm.edu.my/id/eprint/63957/ DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi The origin of gelatin used in gelatin capsule has always been doubtful as it is not always stated in the labelling. Thus, the halal status of the gelatin is questionable and it becomes a great concern by the Muslims. Gelatin is mainly produced from the skin and bones of pig and cow which undergoes either acid or alkali process. Due to the extreme processing conditions, the DNA may encounter high level of degradation. Further processing of the gelatin into gelatin capsule would make it worst. Hence, the minute amount of DNA contained in the gelain and gelatin capsule require careful extraction method and sensitive detection. In this work, the extraction method is optimized in terms of DNA concentration, DNA fragmentation and the presence of real-time PCR inhibitor. Following quality assessment of the DNA extracted, both bovine and porcine are detected using species-specific real-time PCR systems based on SYBR Green reaction.The quality of the DNA extracted from gelatin and gelatin capsules are low in terms of DNA concentration. Using real-time PCR, up to approximately 300 bp target sequence can be amplified and successful detection of both species has been achieved. Halal Products Research Institute, Universiti Putra Malaysia 2014 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63957/1/M38.pdf Mohamad, Nurhidayatul Asma and Khairil Mokhtar, Nur Fadhilah and Mustafa, Shuhaimi (2014) DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR. In: Malaysia International Halal Research & Education Conference 2014 (MIHREC 2014), 2-4 Dec. 2014, Marriott Putrajaya Hotel, Malaysia. (p. 279). |
| spellingShingle | Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR |
| title | DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR |
| title_full | DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR |
| title_fullStr | DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR |
| title_full_unstemmed | DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR |
| title_short | DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR |
| title_sort | dna extraction and species detection in gelatin and gelatin capsule by real-time pcr |
| url | http://psasir.upm.edu.my/id/eprint/63957/ http://psasir.upm.edu.my/id/eprint/63957/1/M38.pdf |