Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopep...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63733/ http://psasir.upm.edu.my/id/eprint/63733/1/Xeno-free%20culture%20of%20human%20pluripotent%20stem%20cells%20on%20oligopeptide-grafted%20hydrogels%20with%20various%20molecular%20designs.pdf |
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| author | Chen, Yen-Ming Chen, Li-Hua Li, Meng-Pei Li, Hsing-Fen Higuchi, Akon Kumar, S. Suresh Ling, Qing-Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Chang, Yung Benelli, Giovanni Murugan, Kadarkarai Umezawa, Akihiro |
| author_facet | Chen, Yen-Ming Chen, Li-Hua Li, Meng-Pei Li, Hsing-Fen Higuchi, Akon Kumar, S. Suresh Ling, Qing-Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Chang, Yung Benelli, Giovanni Murugan, Kadarkarai Umezawa, Akihiro |
| author_sort | Chen, Yen-Ming |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain. Oligopeptide sequences were selected from integrin- and glycosaminoglycan-binding domains of the extracellular matrix. The hydrogels grafted with vitronectin-derived oligopeptides having a joint segment or a dual chain, which has a storage modulus of 25 kPa, supported the long-term culture of human ES and iPS cells for over 10 passages. The dual chain and/or joint segment with cell adhesion molecules on the hydrogels facilitated the proliferation and pluripotency of human ES and iPS cells. |
| first_indexed | 2025-11-15T11:16:31Z |
| format | Article |
| id | upm-63733 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:16:31Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-637332018-11-30T04:21:42Z http://psasir.upm.edu.my/id/eprint/63733/ Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs Chen, Yen-Ming Chen, Li-Hua Li, Meng-Pei Li, Hsing-Fen Higuchi, Akon Kumar, S. Suresh Ling, Qing-Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Chang, Yung Benelli, Giovanni Murugan, Kadarkarai Umezawa, Akihiro Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain. Oligopeptide sequences were selected from integrin- and glycosaminoglycan-binding domains of the extracellular matrix. The hydrogels grafted with vitronectin-derived oligopeptides having a joint segment or a dual chain, which has a storage modulus of 25 kPa, supported the long-term culture of human ES and iPS cells for over 10 passages. The dual chain and/or joint segment with cell adhesion molecules on the hydrogels facilitated the proliferation and pluripotency of human ES and iPS cells. Nature Publishing Group 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63733/1/Xeno-free%20culture%20of%20human%20pluripotent%20stem%20cells%20on%20oligopeptide-grafted%20hydrogels%20with%20various%20molecular%20designs.pdf Chen, Yen-Ming and Chen, Li-Hua and Li, Meng-Pei and Li, Hsing-Fen and Higuchi, Akon and Kumar, S. Suresh and Ling, Qing-Dong and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Chang, Yung and Benelli, Giovanni and Murugan, Kadarkarai and Umezawa, Akihiro (2017) Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs. Scientific Reports, 7. art. no. 45146. pp. 1-16. ISSN 2045-2322 10.1038/srep45146 |
| spellingShingle | Chen, Yen-Ming Chen, Li-Hua Li, Meng-Pei Li, Hsing-Fen Higuchi, Akon Kumar, S. Suresh Ling, Qing-Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Chang, Yung Benelli, Giovanni Murugan, Kadarkarai Umezawa, Akihiro Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| title | Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| title_full | Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| title_fullStr | Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| title_full_unstemmed | Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| title_short | Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| title_sort | xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs |
| url | http://psasir.upm.edu.my/id/eprint/63733/ http://psasir.upm.edu.my/id/eprint/63733/ http://psasir.upm.edu.my/id/eprint/63733/1/Xeno-free%20culture%20of%20human%20pluripotent%20stem%20cells%20on%20oligopeptide-grafted%20hydrogels%20with%20various%20molecular%20designs.pdf |