Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy
Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed 1H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to it...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/63647/ http://psasir.upm.edu.my/id/eprint/63647/1/Urinary%20metabolic%20profiling%20of%20cisplatin%20nephrotoxicity%20and%20nephroprotective%20effects%20of%20Orthosiphon%20stamineus%20leaves%20elucidated%20by%201H%20NMR%20spectroscopy.pdf |
| _version_ | 1848854837769273344 |
|---|---|
| author | Pariyani, Raghunath Ismail, Intan Safinar Azam, Amalina Khatib, Alfi Abas, Faridah Shaari, Khozirah Hamzah, Hazilawati |
| author_facet | Pariyani, Raghunath Ismail, Intan Safinar Azam, Amalina Khatib, Alfi Abas, Faridah Shaari, Khozirah Hamzah, Hazilawati |
| author_sort | Pariyani, Raghunath |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed 1H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to its diuretic and antioxidant activities. Aqueous (OSAE) and 50% aqueous ethanolic (OSFE) extracts of OS leaves were orally administered at 400 mg/kg BW doses to rats which were then intraperitoneally injected with cisplatin at 5 mg/kg BW dose. The 1H NMR profile of the urine samples collected on day 5 after cisplatin administration were analyzed by multivariate pattern recognition techniques, whereby 19 marker metabolites suggestive in the involvement of TCA cycle, disturbed energy metabolism, altered gut microflora and BCAA metabolism pathways were identified. It was observed that OSFE caused significant changes (p < 0.05) in the levels of 8 markers namely leucine, acetate, hippurate, lysine, valine, 2-oxoglutarate, 3-HBT and acetoacetate resulting in a moderate ameliorative effect, however, it did not completely protect from nephrotoxicity. OSAE did not demonstrate significant down regulatory effects on any markers, albeit, it potentiated the cisplatin nephrotoxicity by inducing significant increase in glucose, glycine, creatinine, citrate, TMAO, acetate and creatine levels. A Principal Component Analysis (PCA) of the 1H NMR spectra of OS extracts identified that OSFE had higher concentrations of the secondary metabolites such as caffeic acid, chlorogenic acid, protocatechuic acid and orthosiphol, among others. Whereas, OSAE was characterized by higher concentrations of acetate, lactate, succinic acid, valine and phosphatidylcholine. This research denotes the first comprehensive analysis to identify the effects of OS extracts on cisplatin nephrotoxicity. |
| first_indexed | 2025-11-15T11:16:13Z |
| format | Article |
| id | upm-63647 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:16:13Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-636472018-11-07T08:52:05Z http://psasir.upm.edu.my/id/eprint/63647/ Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy Pariyani, Raghunath Ismail, Intan Safinar Azam, Amalina Khatib, Alfi Abas, Faridah Shaari, Khozirah Hamzah, Hazilawati Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed 1H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to its diuretic and antioxidant activities. Aqueous (OSAE) and 50% aqueous ethanolic (OSFE) extracts of OS leaves were orally administered at 400 mg/kg BW doses to rats which were then intraperitoneally injected with cisplatin at 5 mg/kg BW dose. The 1H NMR profile of the urine samples collected on day 5 after cisplatin administration were analyzed by multivariate pattern recognition techniques, whereby 19 marker metabolites suggestive in the involvement of TCA cycle, disturbed energy metabolism, altered gut microflora and BCAA metabolism pathways were identified. It was observed that OSFE caused significant changes (p < 0.05) in the levels of 8 markers namely leucine, acetate, hippurate, lysine, valine, 2-oxoglutarate, 3-HBT and acetoacetate resulting in a moderate ameliorative effect, however, it did not completely protect from nephrotoxicity. OSAE did not demonstrate significant down regulatory effects on any markers, albeit, it potentiated the cisplatin nephrotoxicity by inducing significant increase in glucose, glycine, creatinine, citrate, TMAO, acetate and creatine levels. A Principal Component Analysis (PCA) of the 1H NMR spectra of OS extracts identified that OSFE had higher concentrations of the secondary metabolites such as caffeic acid, chlorogenic acid, protocatechuic acid and orthosiphol, among others. Whereas, OSAE was characterized by higher concentrations of acetate, lactate, succinic acid, valine and phosphatidylcholine. This research denotes the first comprehensive analysis to identify the effects of OS extracts on cisplatin nephrotoxicity. Elsevier 2017-02-20 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63647/1/Urinary%20metabolic%20profiling%20of%20cisplatin%20nephrotoxicity%20and%20nephroprotective%20effects%20of%20Orthosiphon%20stamineus%20leaves%20elucidated%20by%201H%20NMR%20spectroscopy.pdf Pariyani, Raghunath and Ismail, Intan Safinar and Azam, Amalina and Khatib, Alfi and Abas, Faridah and Shaari, Khozirah and Hamzah, Hazilawati (2017) Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy. Journal of Pharmaceutical and Biomedical Analysis, 135. 20 - 30. ISSN 0731-7085; ESSN: 1873-264X https://www.sciencedirect.com/science/article/pii/S0731708516313292?via%3Dihub 10.1016/j.jpba.2016.12.010 |
| spellingShingle | Pariyani, Raghunath Ismail, Intan Safinar Azam, Amalina Khatib, Alfi Abas, Faridah Shaari, Khozirah Hamzah, Hazilawati Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy |
| title | Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy |
| title_full | Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy |
| title_fullStr | Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy |
| title_full_unstemmed | Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy |
| title_short | Urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of Orthosiphon stamineus leaves elucidated by 1H NMR spectroscopy |
| title_sort | urinary metabolic profiling of cisplatin nephrotoxicity and nephroprotective effects of orthosiphon stamineus leaves elucidated by 1h nmr spectroscopy |
| url | http://psasir.upm.edu.my/id/eprint/63647/ http://psasir.upm.edu.my/id/eprint/63647/ http://psasir.upm.edu.my/id/eprint/63647/ http://psasir.upm.edu.my/id/eprint/63647/1/Urinary%20metabolic%20profiling%20of%20cisplatin%20nephrotoxicity%20and%20nephroprotective%20effects%20of%20Orthosiphon%20stamineus%20leaves%20elucidated%20by%201H%20NMR%20spectroscopy.pdf |