Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta)
Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate t...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63598/ http://psasir.upm.edu.my/id/eprint/63598/1/Transcriptome%20profiling%20of%20sulfate%20deprivation%20responses%20in%20two%20agarophytes%20Gracilaria%20changii%20and%20Gracilaria%20salicornia%20%28Rhodophyta%29.pdf |
| _version_ | 1848854827484839936 |
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| author | Lee, Wei Kang Namasivayam, Parameswari Abdullah, Janna Ong Ho, Chai Ling |
| author_facet | Lee, Wei Kang Namasivayam, Parameswari Abdullah, Janna Ong Ho, Chai Ling |
| author_sort | Lee, Wei Kang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate the effects of sulfate deprivation on the sulfate contents of seaweed and agar, as well as the metabolic pathways of these seaweeds. In this study, two agarophytes G. changii and G. salicornia were treated under sulfate deprivation for 5 days. The sulfate contents in the seaweed/agar were generally lower in sulfate-deprivated samples compared to those in the controls, but the differences were only statistically significant for seaweed sample of G. changii and agar sample of G. salicornia. RNA sequencing (RNA-Seq) of sulfate-deprivated and untreated seaweed samples revealed 1,292 and 3,439 differentially expressed genes (DEGs; ≥1.5-fold) in sulfate-deprivated G. changii and G. salicornia, respectively, compared to their respective controls. Among the annotated DEGs were genes involved in putative agar biosynthesis, sulfur metabolism, metabolism of sulfur-containing amino acids, carbon metabolism and oxidative stress. These findings shed light on the sulfate deprivation responses in agarophytes and help to identify candidate genes involved in agar biosynthesis. |
| first_indexed | 2025-11-15T11:16:03Z |
| format | Article |
| id | upm-63598 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:16:03Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-635982019-10-22T01:35:29Z http://psasir.upm.edu.my/id/eprint/63598/ Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) Lee, Wei Kang Namasivayam, Parameswari Abdullah, Janna Ong Ho, Chai Ling Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate the effects of sulfate deprivation on the sulfate contents of seaweed and agar, as well as the metabolic pathways of these seaweeds. In this study, two agarophytes G. changii and G. salicornia were treated under sulfate deprivation for 5 days. The sulfate contents in the seaweed/agar were generally lower in sulfate-deprivated samples compared to those in the controls, but the differences were only statistically significant for seaweed sample of G. changii and agar sample of G. salicornia. RNA sequencing (RNA-Seq) of sulfate-deprivated and untreated seaweed samples revealed 1,292 and 3,439 differentially expressed genes (DEGs; ≥1.5-fold) in sulfate-deprivated G. changii and G. salicornia, respectively, compared to their respective controls. Among the annotated DEGs were genes involved in putative agar biosynthesis, sulfur metabolism, metabolism of sulfur-containing amino acids, carbon metabolism and oxidative stress. These findings shed light on the sulfate deprivation responses in agarophytes and help to identify candidate genes involved in agar biosynthesis. Nature Publishing Group 2017-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63598/1/Transcriptome%20profiling%20of%20sulfate%20deprivation%20responses%20in%20two%20agarophytes%20Gracilaria%20changii%20and%20Gracilaria%20salicornia%20%28Rhodophyta%29.pdf Lee, Wei Kang and Namasivayam, Parameswari and Abdullah, Janna Ong and Ho, Chai Ling (2017) Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta). Scientific Reports, 7. art. no. 46563. pp. 1-14. ISSN 2045-2322 https://www.nature.com/articles/srep46563 10.1038/srep46563 |
| spellingShingle | Lee, Wei Kang Namasivayam, Parameswari Abdullah, Janna Ong Ho, Chai Ling Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) |
| title | Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) |
| title_full | Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) |
| title_fullStr | Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) |
| title_full_unstemmed | Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) |
| title_short | Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta) |
| title_sort | transcriptome profiling of sulfate deprivation responses in two agarophytes gracilaria changii and gracilaria salicornia (rhodophyta) |
| url | http://psasir.upm.edu.my/id/eprint/63598/ http://psasir.upm.edu.my/id/eprint/63598/ http://psasir.upm.edu.my/id/eprint/63598/ http://psasir.upm.edu.my/id/eprint/63598/1/Transcriptome%20profiling%20of%20sulfate%20deprivation%20responses%20in%20two%20agarophytes%20Gracilaria%20changii%20and%20Gracilaria%20salicornia%20%28Rhodophyta%29.pdf |